Nikolin Veljko M, Donofrio Gaetano, Milosevic Bratislav, Taddei Simone, Radosavljevic Vladimir, Milicevic Vesna
Department of Virology, The Institute of Veterinary Medicine of Serbia, Belgrade, Belgrade, Serbia.
New Microbiol. 2007 Jan;30(1):53-7.
Two farms in the Belgrade area have experienced serious problems with postpartal metritis. Serological examination of BoHV-4 infection was done using ELISA test and vaginal swabs were used for virus isolation. Average seroprevalence of BoHV-4 in these farms was 84.37%. BoHV-4 isolation was successful from three vaginal swabs on the MDBK cell line. Rising values of BoHV-4 antibodies were recorded in nine of ten cows with clinical signs of postpartal metritis. PCR and restriction analysis were used for better characterisation and isolate classification. Two isolates showed similarity with MOVAR 33/63 virus type, but one differed in polyrepetitive and other parts of DNA. This was the first isolation and characterisation of BoHV4 from Serbian herds.
贝尔格莱德地区的两个农场出现了严重的产后子宫炎问题。使用酶联免疫吸附测定（ELISA）试验对牛疱疹病毒4型（BoHV - 4）感染进行了血清学检查，并使用阴道拭子进行病毒分离。这些农场中BoHV - 4的平均血清阳性率为84.37%。在MDBK细胞系上，从三份阴道拭子中成功分离出BoHV - 4。在十头有产后子宫炎临床症状的奶牛中，有九头的BoHV - 4抗体值呈上升趋势。采用聚合酶链反应（PCR）和限制性分析进行更好的特征鉴定和分离株分类。两个分离株与MOVAR 33/63病毒型相似，但有一个在DNA的多重复序列和其他部分有所不同。这是首次从塞尔维亚牛群中分离和鉴定出BoHV4。
