Department of Electrical and Computer Engineering, 161 College Avenue, Medford, MA 02155, USA.
Biosens Bioelectron. 2014 Nov 15;61:625-30. doi: 10.1016/j.bios.2014.05.067. Epub 2014 Jun 9.
Here we present a high-throughput, transparent microfluidic device with embedded microwell arrays sandwiched between transparent electrodes made from graphene (at the bottom) and indium tin oxide (at the top) for dielectrophoretic cell trapping and electrical lysis. Graphene suppresses unwanted faradaic reaction effects on the cells and the medium that is typically observed in ITO based electrodes from application of DC field for electrical lysis. This is because graphene is more electrochemically inert than indium tin oxide (ITO) where ITO undergoes reduction-oxidation (redox) reaction in the presence of electrolyte in most standard cell media. This redox process also compromises ITO's electrical properties and optical transparency over multiple use. The presented microfluidic device shows high efficiency for cell trapping and lysis and an electrochemically stable behavior for long operational life.
在这里，我们展示了一种高通量、透明的微流控装置，其中嵌入了微井阵列，夹在由石墨烯（底部）和氧化铟锡（顶部）制成的透明电极之间，用于介电泳细胞捕获和电裂解。石墨烯抑制了在基于 ITO 的电极中观察到的、通常由施加直流电场进行电裂解引起的对细胞和介质的不需要的法拉第反应效应。这是因为石墨烯比氧化铟锡（ITO）更具有电化学惰性，在大多数标准细胞培养基中，ITO 在电解质存在下会发生还原-氧化（氧化还原）反应。这个氧化还原过程也会影响 ITO 的电性能和光学透明度，使其在多次使用后性能下降。所提出的微流控装置显示出高效的细胞捕获和裂解效率，以及电化学稳定的长使用寿命行为。
