Li Lianhuang, Jiang Weizhong, Yang Yinghong, Chen Zhifen, Feng Changyin, Li Hongsheng, Guan Guoxian, Chen Jianxin
Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fuzhou 350007, China.
Fujian Medical University, The Affiliated Union Hospital, Department of Colorectal Surgery, Fuzhou 350001, China.
J Biomed Opt. 2014 Jun;19(6):066008. doi: 10.1117/1.JBO.19.6.066008.
Dirty necrosis within glandular lumina is often considered as a characteristic of colorectal carcinomas (CRCs) that is a diagnostically useful feature of CRCs with DNA microsatellite instability (MSI). Multiphoton microscopy (MPM), which is based on the second-harmonic generation and two-photon excited fluorescence signals, was used to identify dirty necrosis. Our results demonstrated that MPM has the ability to exhibit the microstructure of dirty necrosis and the signal intensity as well as an emission spectrum that can help to differentiate dirty necrosis from cancer cells. These findings indicate that MPM may be helpful in distinguishing MSI colorectal carcinoma via the identification of dirty necrosis.
腺腔内的脏坏死通常被认为是结直肠癌（CRC）的一个特征，这是具有DNA微卫星不稳定性（MSI）的CRC的一个诊断有用特征。基于二次谐波产生和双光子激发荧光信号的多光子显微镜（MPM）被用于识别脏坏死。我们的结果表明，MPM有能力展现脏坏死的微观结构、信号强度以及有助于将脏坏死与癌细胞区分开来的发射光谱。这些发现表明，MPM可能有助于通过识别脏坏死来区分MSI结直肠癌。
