Santos Cátia, Lucas Marco S, Dias Albino A, Bezerra Rui M F, Peres José A, Sampaio Ana
Centro de Investigação e de Tecnologias Agro-ambientais e Biológicas (CITAB), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta dos Prados, 5001-801 Vila Real, Portugal.
Centro de Química de Vila Real (CQVR), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta dos Prados, 5001-801 Vila Real, Portugal.
Chemosphere. 2014 Dec;117:53-8. doi: 10.1016/j.chemosphere.2014.05.083. Epub 2014 Jun 24.
Winery wastewaters (WW) have high levels of organic matter, resulting in high COD and BOD and suspended solids. This paper studies the combination of biological and chemical processes in WW treatment. Among 10 yeast isolates, Filobasidium sp. (AGG 577) and Cryptococcus laurentii (AGG 726) were selected due to their superior performance in COD removal. During WW degradation, COD and total polyphenols (TPP) content removal of 89-90% for Filobasidium sp. and 90-93% for C. laurentii were obtained. However, despite similar degradation efficiency for both yeasts, COD kinetics and pH evolution during treatment reveals that C. laurentii presents a faster response than Filobasidium sp. The toxicity (inhibition of Vibrio fischeri luminescence) of C. laurentii treated WW decreases to an inhibition value below 2.5%. However, treated WW exceeds the legal limits, making necessary an additional treatment. In this case, the selection of Fenton's reagent as a chemical final polish step process is a good compromise between efficiency and lower practical complexity. The best results for both COD and TPP removal were obtained with H2O2 initial concentration of 39.2mM and a H2O2:Fe(2+) molar ratio of 15:1. The combined C. laurentii - Fenton's reagent treatment of WW achieved a total reduction of 98% and 96%, for COD and TPP, respectively.
葡萄酒厂废水（WW）含有大量有机物，导致化学需氧量（COD）、生化需氧量（BOD）和悬浮物含量很高。本文研究了葡萄酒厂废水处理中生物和化学工艺的结合。在10株酵母分离株中，由于丝状担子菌（AGG 577）和罗伦隐球酵母（AGG 726）在COD去除方面表现优异而被选中。在葡萄酒厂废水降解过程中，丝状担子菌的COD和总多酚（TPP）含量去除率达到89 - 90%，罗伦隐球酵母的去除率为90 - 93%。然而，尽管两种酵母的降解效率相似，但处理过程中的COD动力学和pH值变化表明，罗伦隐球酵母的反应比丝状担子菌更快。经罗伦隐球酵母处理的废水毒性（对费氏弧菌发光的抑制作用）降至2.5%以下的抑制值。然而，处理后的废水仍超过法定限值，因此需要进行额外处理。在这种情况下，选择芬顿试剂作为化学最终精处理步骤在效率和较低的实际复杂性之间是一个很好的折衷方案。当过氧化氢初始浓度为39.2mM且过氧化氢与亚铁离子的摩尔比为15:1时，COD和TPP去除效果最佳。罗伦隐球酵母 - 芬顿试剂联合处理葡萄酒厂废水使COD和TPP的总去除率分别达到98%和96%。
