Winterroth Frank, Kato Hiroko, Kuo Shiuhyang, Feinberg Stephen E, Hollister Scott J, Fowlkes J Brian, Hollman Kyle W
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA.
Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA; Department of Oral Anatomy, Course for Oral Life Science, Niigata University Postgraduate School of Medical and Dental Sciences, Niigata, Japan.
Ultrasound Med Biol. 2014 Sep;40(9):2244-51. doi: 10.1016/j.ultrasmedbio.2014.03.007. Epub 2014 Jun 23.
This study uses high-resolution ultrasound to examine the growth and development of engineered oral mucosal tissues manufactured under aseptic conditions. The specimens are a commercially available natural tissue scaffold, AlloDerm, and oral keratinocytes seeded onto AlloDerm to form an ex vivo-produced oral mucosal equivalent (EVPOME) suitable for intra-oral grafting. The seeded cells produce a keratinized protective upper layer that smooths out any remaining surface irregularities on the underlying AlloDerm. Two-dimensional acoustic imaging of unseeded AlloDerm and developing EVPOMEs was performed on each day of their growth and development, each tissue specimen being imaged under aseptic conditions (total time from seeding to maturation: 11 d). Ultrasonic monitoring offers us the ability to determine the constituents of the EVPOME that are responsible for changes in its mechanical behavior during the manufacturing process. Ultrasonic monitoring affords us an opportunity to non-invasively assess, in real time, tissue-engineered constructs before release for use in patient care.
本研究使用高分辨率超声检查在无菌条件下制造的工程化口腔黏膜组织的生长和发育情况。样本包括一种市售的天然组织支架——脱细胞异体真皮基质（AlloDerm），以及接种到AlloDerm上的口腔角质形成细胞，以形成适合口腔内移植的体外产生的口腔黏膜等效物（EVPOME）。接种的细胞产生角质化的保护性上层，使下层AlloDerm上任何残留的表面不规则处变得平滑。在未接种的AlloDerm和发育中的EVPOME生长发育的每一天，都对其进行二维声学成像，每个组织样本均在无菌条件下成像（从接种到成熟的总时间：11天）。超声监测使我们有能力确定EVPOME中在制造过程中导致其力学行为发生变化的成分。超声监测为我们提供了一个机会，可以在用于患者护理之前，对组织工程构建体进行实时、非侵入性评估。
