Conconi Maria Teresa, Borgio Luca, Di Liddo Rosa, Sartore Leonardo, Dalzoppo Daniele, Amistà Pietro, Lora Silvano, Parnigotto Pier Paolo, Grandi Claudio
Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua 35131, Italy.
Department of Plastic and Reconstructive Surgery, University of Padua, Padua 35131, Italy.
Mol Med Rep. 2014 Sep;10(3):1329-34. doi: 10.3892/mmr.2014.2348. Epub 2014 Jun 19.
The present study designed and developed blood vessel substitutes (BVSs) composed of polyvinyl alcohol (PVA) cryogels. The in vitro results demonstrated that the coating of the polymer with lyophilized decellularized vascular matrix (DVM) greatly enhanced the adhesion of human umbilical vein endothelial cells (HUVECs). However, when PVA̸DVM BVSs were implanted into the abdominal aorta of Sprague‑Dawley rats, DVM was identified as a highly thrombogenic surface resulting in the mortality of all animals 3‑4 days after surgery. By contrast, all rats implanted with PVA survived and were sacrificed after 12 months. The luminal surface of the explanted grafts was completely covered by endothelial cells and the inner diameter was similar to that of the original vessel. In conclusion, the present study indicated that PVA may be considered as a promising biomaterial for the fabrication of artificial vessels.
本研究设计并开发了由聚乙烯醇（PVA）冷冻凝胶组成的血管替代物（BVSs）。体外实验结果表明，用冻干的脱细胞血管基质（DVM）对聚合物进行包被可显著增强人脐静脉内皮细胞（HUVECs）的黏附。然而，当将PVA/DVM BVSs植入Sprague-Dawley大鼠的腹主动脉时，DVM被认为是具有高度血栓形成倾向的表面，导致所有动物在术后3-4天死亡。相比之下，所有植入PVA的大鼠均存活，并在12个月后被处死。取出的移植物管腔表面完全被内皮细胞覆盖，内径与原始血管相似。总之，本研究表明PVA可被视为一种用于制造人造血管的有前景的生物材料。
