[镍钛形状记忆合金与不锈钢激光焊接的体外细胞毒性检测]

[Cytotoxicitic detection of laser welding between NiTi shape memory alloy and stainless steel in vitro].

作者信息

Yu Wenwen, Zhang Chao, Zhang Bing, Liu Jiming, Sun Xinhua

机构信息

Department of Orthodontics, School of Stomatology, Jilin University, Changchun 130021, China.

Department of Orthodontics, School of Stomatology, Jilin University, Changchun 130021, China. Email:

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2014 Apr;49(4):239-43.

PMID:24969600

Abstract

OBJECTIVE

To investigate the cytotoxicity of laser-welded nickel titanium (NiTi) and stainless steel composite archwire.

METHODS

The NiTi and stainless steel composite archwire (CoAW) laser-welded with pure copper inrerplayer was studied with methyl thiazolyl tetrazolium (MTT) test in vitro. The cytotoxicity of CoAW was compared with stainless steel archwire and NiTi archwire. Two tests were carried out. Test 1: the immersed solution of CoAW was diluted to five grades (50%, 40%, 30%, 20%, 10%). The cytotoxicity in vitro of these agents was assayed on murine fibroblast cell L929 line with MTT test at 24 and 48 hours. Test 2: the immeresed solution of CoAW, NiTi archwires and stainless steel archwires was diluted to four grads (100%, 75%, 50%, 25%). The cytotoxity of three kinds of material was compared at 48 hours.

RESULTS

The results of all samples revealed level 0-1 cytotoxicity. In test 1, the same grade solution optical density (except 20%) at 24 hours was statistically lower than at 48 hours. In test 2, the optical density of CoAW solution (1.964 ± 0.122, 2.084 ± 0.056, 2.056 ± 0.071, 2.096 ± 0.050) was statistically lower than the same grade solution of stainless steel archwire (2.168 ± 0.091, 2.227 ± 0.160, 2.302 ± 0.052, 2.301 ± 0.060) and NiTi archwire (2.138 ± 0.105, 2.262 ± 0.050, 2.271 ± 0.082, 2.294 ± 0.056) (P < 0.05).

CONCLUSIONS

The MTT test of CoAW in vitro showed that cytotoxicity was related to concentration and time. The cytotoxicity of the CoAW was more serious than that of stainless steel and NiTi archwires. However, CoAW belonged to secure rang of material toxicity reaction.

摘要

目的

研究激光焊接镍钛（NiTi）与不锈钢复合弓丝的细胞毒性。

方法

采用甲基噻唑基四氮唑（MTT）体外试验研究镍钛与不锈钢复合弓丝（CoAW）与纯铜内衬激光焊接后的情况。将CoAW的细胞毒性与不锈钢弓丝和镍钛弓丝进行比较。进行了两项试验。试验1：将CoAW的浸泡液稀释为五个浓度等级（50%、40%、30%、20%、10%）。在24小时和48小时时，用MTT试验检测这些制剂对小鼠成纤维细胞L929系的体外细胞毒性。试验2：将CoAW、镍钛弓丝和不锈钢弓丝的浸泡液稀释为四个浓度等级（100%、75%、50%、25%）。在48小时时比较三种材料的细胞毒性。

结果

所有样本结果显示细胞毒性为0 - 1级。在试验1中，相同浓度溶液在24小时时的光密度（20%浓度除外）统计学上低于48小时时。在试验2中，CoAW溶液的光密度（1.964±0.122、2.084±0.056、2.056±0.071、2.096±0.050）统计学上低于不锈钢弓丝（2.168±0.091、2.227±0.160、2.302±0.052、2.301±0.060）和镍钛弓丝（2.138±0.105、2.262±0.050、2.271±0.082、2.294±0.056）的相同浓度溶液（P<0.05）。