• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

对映互补的解脂耶氏酵母氧化还原酶：醇脱氢酶 2 和短链脱氢酶/还原酶。

Enantiocomplementary Yarrowia lipolytica Oxidoreductases: Alcohol Dehydrogenase 2 and Short Chain Dehydrogenase/Reductase.

机构信息

ACIB (Austrian Centre of Industrial Biotechnology) GmbH, Petersgasse 14/III, Graz 8010, Austria.

LONZA AG, Rottenstrasse 6, Visp 3930, Switzerland.

出版信息

Biomolecules. 2013 Aug 12;3(3):449-60. doi: 10.3390/biom3030449.

DOI:10.3390/biom3030449
PMID:24970175
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4030946/
Abstract

Enzymes of the non-conventional yeast Yarrowia lipolytica seem to be tailor-made for the conversion of lipophilic substrates. Herein, we cloned and overexpressed the Zn-dependent alcohol dehydrogenase ADH2 from Yarrowia lipolytica in Escherichia coli. The purified enzyme was characterized in vitro. The substrate scope for YlADH2 mediated oxidation and reduction was investigated spectrophotometrically and the enzyme showed a broader substrate range than its homolog from Saccharomyces cerevisiae. A preference for secondary compared to primary alcohols in oxidation direction was observed for YlADH2. 2-Octanone was investigated in reduction mode in detail. Remarkably, YlADH2 displays perfect (S)-selectivity and together with a highly (R)-selective short chain dehydrogenase/ reductase from Yarrowia lipolytica it is possible to access both enantiomers of 2-octanol in >99% ee with Yarrowia lipolytica oxidoreductases.

摘要

非传统酵母解脂耶氏酵母的酶似乎是专门为转化亲脂性底物而设计的。在此，我们在大肠杆菌中克隆并过表达了来自解脂耶氏酵母的 Zn 依赖性醇脱氢酶 ADH2。体外纯化了该酶，并通过分光光度法研究了 YlADH2 介导的氧化和还原的底物范围，结果表明该酶的底物范围比其来自酿酒酵母的同源物更广泛。在氧化方向上，YlADH2 优先选择仲醇而不是伯醇。详细研究了 2-辛酮在还原模式下的情况。值得注意的是，YlADH2 表现出完美的 (S)-选择性，并且与来自解脂耶氏酵母的高 (R)-选择性短链脱氢酶/还原酶一起，使用解脂耶氏酵母氧化还原酶可以以 >99%ee 获得 2-辛醇的两种对映体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30f9/4030946/330deadf6cb0/biomolecules-03-00449-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30f9/4030946/d85032ec79ba/biomolecules-03-00449-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30f9/4030946/e9991583875a/biomolecules-03-00449-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30f9/4030946/bd950827d8f3/biomolecules-03-00449-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30f9/4030946/330deadf6cb0/biomolecules-03-00449-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30f9/4030946/d85032ec79ba/biomolecules-03-00449-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30f9/4030946/e9991583875a/biomolecules-03-00449-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30f9/4030946/bd950827d8f3/biomolecules-03-00449-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30f9/4030946/330deadf6cb0/biomolecules-03-00449-g004.jpg

相似文献

1
Enantiocomplementary Yarrowia lipolytica Oxidoreductases: Alcohol Dehydrogenase 2 and Short Chain Dehydrogenase/Reductase.对映互补的解脂耶氏酵母氧化还原酶：醇脱氢酶 2 和短链脱氢酶/还原酶。
Biomolecules. 2013 Aug 12;3(3):449-60. doi: 10.3390/biom3030449.
2
Yarrowia lipolytica dehydrogenase/reductase: an enzyme tolerant for lipophilic compounds and carbohydrate substrates.解脂耶氏酵母醇脱氢酶/还原酶：一种对亲脂性化合物和碳水化合物底物具有耐受性的酶。
Bioorg Med Chem Lett. 2013 Jun 1;23(11):3393-5. doi: 10.1016/j.bmcl.2013.03.064. Epub 2013 Apr 6.
3
Three alcohol dehydrogenase genes and one acetyl-CoA synthetase gene are responsible for ethanol utilization in Yarrowia lipolytica.三个乙醇脱氢酶基因和一个乙酰辅酶A合成酶基因负责解脂耶氏酵母中的乙醇利用。
Fungal Genet Biol. 2016 Oct;95:30-38. doi: 10.1016/j.fgb.2016.07.012. Epub 2016 Jul 30.
4
A newly identified fatty alcohol oxidase gene is mainly responsible for the oxidation of long-chain ω-hydroxy fatty acids in Yarrowia lipolytica.一个新鉴定出的脂肪醇氧化酶基因主要负责解脂耶氏酵母中长链ω-羟基脂肪酸的氧化。
FEMS Yeast Res. 2014 Sep;14(6):858-72. doi: 10.1111/1567-1364.12176. Epub 2014 Jul 2.
5
Enantioselective synthesis of enantiopure chiral alcohols using carbonyl reductases screened from Yarrowia lipolytica.利用从解脂假丝酵母中筛选出的羰基还原酶对映选择性合成手性纯手性醇。
J Appl Microbiol. 2019 Jan;126(1):127-137. doi: 10.1111/jam.14125. Epub 2018 Oct 29.
6
Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts and .工程酵母中脂肪醇生产的多组学分析及…… （原文不完整）
Front Genet. 2019 Aug 30;10:747. doi: 10.3389/fgene.2019.00747. eCollection 2019.
7
Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica.利用产油酵母解脂耶氏酵母可持续生产脂肪酸乙酯生物柴油。
Microbiologyopen. 2020 Jul;9(7):e1051. doi: 10.1002/mbo3.1051. Epub 2020 Apr 27.
8
Understanding Functional Roles of Native Pentose-Specific Transporters for Activating Dormant Pentose Metabolism in Yarrowia lipolytica.了解天然戊糖特异性转运蛋白在激活解脂耶氏酵母休眠戊糖代谢中的功能作用。
Appl Environ Microbiol. 2018 Jan 17;84(3). doi: 10.1128/AEM.02146-17. Print 2018 Feb 1.
9
Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis.解脂耶氏酵母中赤藓糖还原酶的特性及其对赤藓糖醇合成的影响。
Microb Cell Fact. 2017 Jul 11;16(1):118. doi: 10.1186/s12934-017-0733-6.
10
Alcohol dehydrogenases and an alcohol oxidase involved in the assimilation of exogenous fatty alcohols in Yarrowia lipolytica.参与解脂耶氏酵母中外源脂肪醇同化作用的乙醇脱氢酶和一种乙醇氧化酶。
FEMS Yeast Res. 2015 May;15(3). doi: 10.1093/femsyr/fov014. Epub 2015 Mar 23.

引用本文的文献

1
Validated Growth Rate-Dependent Regulation of Lipid Metabolism in .在. 中验证了脂质代谢的生长速率依赖性调节。
Int J Mol Sci. 2022 Jul 31;23(15):8517. doi: 10.3390/ijms23158517.

本文引用的文献

1
Yarrowia lipolytica dehydrogenase/reductase: an enzyme tolerant for lipophilic compounds and carbohydrate substrates.解脂耶氏酵母醇脱氢酶/还原酶：一种对亲脂性化合物和碳水化合物底物具有耐受性的酶。
Bioorg Med Chem Lett. 2013 Jun 1;23(11):3393-5. doi: 10.1016/j.bmcl.2013.03.064. Epub 2013 Apr 6.
2
Biochemical and structural characterization of recombinant short-chain NAD(H)-dependent dehydrogenase/reductase from Sulfolobus acidocaldarius highly enantioselective on diaryl diketone benzil.短链 NAD(H)-依赖性脱氢酶/还原酶的重组 Sulfolobus acidocaldarius 的生化和结构特征，对二芳基二酮苯甲酰具有高度对映选择性。
Appl Microbiol Biotechnol. 2013 May;97(9):3949-64. doi: 10.1007/s00253-012-4273-z. Epub 2012 Jul 18.
3
Host cell and expression engineering for development of an E. coli ketoreductase catalyst: enhancement of formate dehydrogenase activity for regeneration of NADH.
用于开发大肠杆菌酮还原酶催化剂的宿主细胞和表达工程：增强甲酸脱氢酶活性以再生 NADH。
Microb Cell Fact. 2012 Jan 11;11:7. doi: 10.1186/1475-2859-11-7.
4
The alcohol dehydrogenase system in the xylose-fermenting yeast Candida maltosa.木糖发酵酵母 Candida maltosa 中的醇脱氢酶系统。
PLoS One. 2010 Jul 23;5(7):e11752. doi: 10.1371/journal.pone.0011752.
5
Characterization of alcohol dehydrogenase 1 of the thermotolerant methylotrophic yeast Hansenula polymorpha.热耐受甲基营养酵母汉逊德巴利酵母醇脱氢酶 1 的特性。
Appl Microbiol Biotechnol. 2010 Sep;88(2):497-507. doi: 10.1007/s00253-010-2752-7. Epub 2010 Jul 16.
6
Production of glucolipids and specialty fatty acids from sophorolipids by Penicillium decumbens naringinase: Optimization and kinetics.扩展青霉柚皮苷酶催化槐糖脂生成糖脂和特种脂肪酸：优化及动力学研究
Biotechnol J. 2009 Apr;4(4):517-24. doi: 10.1002/biot.200800209.
7
A simple and efficient expression and purification system using two newly constructed vectors.一种使用两个新构建载体的简单高效的表达和纯化系统。
Protein Expr Purif. 2009 Feb;63(2):102-11. doi: 10.1016/j.pep.2008.09.008. Epub 2008 Sep 20.
8
Biocatalytic reductions: from lab curiosity to "first choice".生物催化还原：从实验室的新奇事物到“首选”。
Acc Chem Res. 2007 Dec;40(12):1260-6. doi: 10.1021/ar7001073. Epub 2007 Oct 18.
9
(S)-(+)-2-octanol as a chiral oil core for the microemulsion electrokinetic chromatographic separation of chiral basic drugs.
Anal Sci. 2007 Sep;23(9):1071-5. doi: 10.2116/analsci.23.1071.
10
Crystal structure and biochemical properties of the D-arabinose dehydrogenase from Sulfolobus solfataricus.嗜热栖热菌D-阿拉伯糖脱氢酶的晶体结构和生化特性
J Mol Biol. 2007 Aug 31;371(5):1249-60. doi: 10.1016/j.jmb.2007.05.097. Epub 2007 Jun 8.