Mandal Sangeeta, Jena J K, Singh Rajeev K, Mohindra Vindhya, Lakra W S, Deshmukhe Geetanjali, Kumar Rajesh, Lal Kuldeep K
a Division of Fish Conservation , National Bureau of Fish Genetic Resources , Lucknow-226002 , UP , India and.
b Fisheries Resources, Harvest and Post Harvest Division, Central Institute of Fisheries Education , Mumbai-400061 , India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1075-9. doi: 10.3109/19401736.2014.928874. Epub 2014 Jun 27.
Silonia silondia is a commercially important food fish. Samples collected through commercial catches from four rivers in India are described by sequence analysis of two molecular markers. Cytochrome b (1140 bp) and ATPase 6/8 (842 bp) genes were analyzed, which represented high level of genetic differentiation within populations of S. silondia. The sequence alignments of cytochrome b and ATPase 6/8 genes revealed 13 and 11 different haplotypes, respectively. The sequences of both the mitochondrial regions revealed high haplotype and low nucleotide diversities. The patterns of genetic diversity and haplotype networks clearly indicated two distinct mitochondrial lineages, however, haplotypes from both the lineages were not specifically assigned to any population. The results confirm the utility of molecular markers generating baseline information, useful for planning effective strategies for conservation, management and sustainability of Silond catfish fishery.
席隆底鳢是一种具有重要商业价值的食用鱼类。通过对印度四条河流的商业捕捞所收集的样本,利用两种分子标记进行序列分析来描述。分析了细胞色素b(1140bp)和ATP酶6/8(842bp)基因,这些基因在席隆底鳢种群中表现出高水平的遗传分化。细胞色素b和ATP酶6/8基因的序列比对分别揭示了13种和11种不同的单倍型。两个线粒体区域的序列均显示出高单倍型多样性和低核苷酸多样性。遗传多样性模式和单倍型网络清楚地表明存在两个不同的线粒体谱系,然而,来自两个谱系的单倍型并未明确归属于任何一个种群。结果证实了分子标记在生成基线信息方面的效用,这对于规划席隆底鳢渔业的有效保护、管理和可持续发展策略很有用。