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基于ATP酶8/6基因的条纹鳢(鲈形目,鳢科)遗传多样性评估

ATPase 8/6 GENE BASED GENETIC DIVERSITY ASSESSMENT OF SNAKEHEAD MURREL, Channa striata (Perciformes, Channidae).

作者信息

Baisvar V S, Kumar R, Singh M, Singh A K, Chauhan U K, Nagpure N S, Kushwaha B

出版信息

Genetika. 2015 Oct;51(10):1171-83. doi: 10.7868/s0016675815100045.

DOI:10.7868/s0016675815100045
PMID:27169232
Abstract

The mitochondrial DNA (mtDNA) ATPase 8/6 gene has been used in phylogenetic as well as in phylogeographic studies along with other mtDNA markers. In this study, ATPase gene sequences were used to assess the genetic structuring and phylogeographic patterns in Channa striata. Out of 884 nucleotide positions generated in ATPase 8/6 genes, 76 were polymorphic. The study suggested 23 unique haplotypes from 67 individuals of nine populations collected from different riverine systems of India. The ATPase 8/6 sequence revealed highest haplotype as well as nucleotide diversities in Imphal River population and lowest diversities in Tapti River population. The pattern of genetic diversity and haplotype network indicated distinct mitochondrial lineages for Chaliyar population, whereas mismatch distribution strongly suggested a population expansion in mid pleistocene epoch (0.4 Mya) with distinct genetic structuring in C. striata. The baseline information on genetic variation and the population sub-structuring would facilitate conservation and management of this important snakehead murrel.

摘要

线粒体DNA(mtDNA)ATP酶8/6基因已与其他mtDNA标记一起用于系统发育和系统地理学研究。在本研究中,ATP酶基因序列被用于评估纹鳢的遗传结构和系统地理模式。在ATP酶8/6基因产生的884个核苷酸位置中,有76个是多态性的。该研究从印度不同河流系统采集的9个种群的67个个体中发现了23种独特的单倍型。ATP酶8/6序列显示因帕尔河种群的单倍型和核苷酸多样性最高,而塔普蒂河种群的多样性最低。遗传多样性模式和单倍型网络表明查利亚尔种群有独特的线粒体谱系,而失配分布强烈表明在更新世中期(0.4百万年前)有一次种群扩张,且纹鳢有明显的遗传结构。关于遗传变异和种群亚结构的基线信息将有助于对这种重要的鳢科鱼类进行保护和管理。

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