Zeng Mei, Chen Baofeng, Qing Yufeng, Xie Wenguang, Dang Wantai, Zhao Mingcai, Zhou Jingguo
a Biology Group, North Sichuan Medical College.
Nucleosides Nucleotides Nucleic Acids. 2014;33(7):455-65. doi: 10.1080/15257770.2014.885045.
Glut9 is highly expressed in the human kidney proximal convoluted tubular and plays a crucial role in the regulation of plasma urate levels. The gene effects were stronger among women. Our results show that 17-β-estradiol (E2) through ER (estrogen receptor) β downregulates Glut9 protein expression on human renal tubular epithelial cell line (HK2). Intriguingly, E2 does not affect the expression of Glut9 mRNA. ERβ is linked to PTEN, the PTEN gene negatively regulates the PI3K/AKT pathway, and the PI3K/AKT pathway inhibition may lead to autophagy. Further study indicates that ERβ may affect the expression of Glut9 though autophagy.
Glut9在人肾近端曲管中高度表达，在血浆尿酸水平调节中起关键作用。基因效应在女性中更强。我们的结果表明，17-β-雌二醇（E2）通过雌激素受体（ER）β下调人肾小管上皮细胞系（HK2）上的Glut9蛋白表达。有趣的是，E2不影响Glut9 mRNA的表达。ERβ与PTEN相关联，PTEN基因负调节PI3K/AKT途径，并且PI3K/AKT途径的抑制可能导致自噬。进一步研究表明，ERβ可能通过自噬影响Glut9的表达。
