Sheng Shifeng, Chen Jingfeng, Zhang Yuheng, Qin Qian, Li Weikang, Yan Su, Wang Youxiang, Li Tiantian, Gao Xinxin, Tang Lin, Li Ang, Ding Suying
Health Management Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
College of Public Health, Zhengzhou University, Zhengzhou, China.
Front Med (Lausanne). 2021 Nov 19;8:779994. doi: 10.3389/fmed.2021.779994. eCollection 2021.
To investigate the correlation between the structure and function alterations of gut microbiota and biochemical indicators in males with hyperuricemia (HUA) and high levels of liver enzymes, in order to provide new evidences and therapeutic targets for the clinical diagnosis and treatment of HUA. A total of 69 patients with HUA (HUA group) and 118 healthy controls were enrolled in this study. Their age, height, waist circumference, weight, and pressure were measured. The clinical parameters such as fasting plasma glucose (FBG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum uric acid (SUA), serum creatinine (Scr), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), white blood cell (WBC), platelet (PLT), and absolute value of neutrophils (NEUT) were examined. We used whole-genome shotgun sequencing technology and HUMAnN2 MetaCyc pathway database to detect the composition and pathways of the gut microbiota. The main statistical methods were student's test, chi-square tests, and Wilcoxon rank sum test. The correlations among bacterial diversity, microbial pathways, and biochemical indicators were evaluated by the R function "cor.test" with spearman method. The gut bacterial diversity in HUA group reduced significantly and the community of the microbiota was of significant difference between the two groups. The pathways that can produce 5-aminoimidazole ribonucleotide (PWY-6122, PWY-6277, and PWY-6121), aromatic amino acids, and chorismate (COMPLETE-ARO-PWY, ARO-PWY, and PWY-6163) were enriched in the HUA group; while the pathways that can produce short-chain fatty acids (SCFAs, such as CENTFERM-PWY and PWY-6590) and the gut microbiotas that can produce SCFAs () and equol () were enriched in healthy controls. The structure and function of the gut microbiota in males with HUA and high levels of liver enzymes have altered apparently. In-depth study of related mechanisms may provide new ideas for the treatment of HUA.
为探讨高尿酸血症（HUA）及肝酶水平升高男性肠道微生物群结构与功能改变与生化指标之间的相关性，为HUA的临床诊断和治疗提供新的证据及治疗靶点。本研究共纳入69例HUA患者（HUA组）和118例健康对照者。测量他们的年龄、身高、腰围、体重和血压。检测空腹血糖（FBG）、天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、血清尿酸（SUA）、血清肌酐（Scr）、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、白细胞（WBC）、血小板（PLT）及中性粒细胞绝对值（NEUT）等临床参数。采用全基因组鸟枪法测序技术和HUMAnN2 MetaCyc通路数据库检测肠道微生物群的组成和通路。主要统计方法为t检验、卡方检验和Wilcoxon秩和检验。采用R函数“cor.test”的spearman方法评估细菌多样性、微生物通路与生化指标之间的相关性。HUA组肠道细菌多样性显著降低，两组微生物群落存在显著差异。HUA组中可产生5-氨基咪唑核糖核苷酸的通路（PWY-6122、PWY-6277和PWY-6121）、芳香族氨基酸和分支酸的通路（COMPLETE-ARO-PWY、ARO-PWY和PWY-6163）富集；而可产生短链脂肪酸的通路（如CENTFERM-PWY和PWY-6590）以及可产生短链脂肪酸（）和雌马酚（）的肠道微生物群在健康对照者中富集。HUA及肝酶水平升高男性的肠道微生物群结构和功能明显改变。深入研究相关机制可能为HUA的治疗提供新思路。
