He Jian-Rong, Liu Xue-Ying, Wang Qing-Wei, Ye Zi-Chen, Chang Min
Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Apr;31(4):711-3.
To establish a reversed-phase high-performance liquid chromatographic (RP-HPLC) method for determination of phloridzin content.
A RP-HPLC method was established for determination of phloridzin using an Inertsil ODS-3 (4.6×150 mm, 5 µm) column with the detection wavelength of 288 nm, flow rate of 1.0 ml/min, and column temperature of 25 degrees celsius;.
The result showed that the phloridzin had a good linear relationship when its concentration ranged between 0.5988 and 89.72 µg/ml. The regression equation was Y=46.370 X-0.6728 (r=0.9999, n=3). The average recovery of phloridzin was 99.40% with the relative standard deviations (RSD) of 0.67%.
This method is simple, quick and accurate for determination of phloridzin content.
建立一种反相高效液相色谱（RP-HPLC）法测定根皮苷含量。
采用Inertsil ODS-3（4.6×150 mm，5 µm）色谱柱，检测波长为288 nm，流速为1.0 ml/min，柱温为25℃，建立RP-HPLC法测定根皮苷含量。
结果表明，根皮苷浓度在0.5988至89.72 µg/ml之间时具有良好的线性关系。回归方程为Y = 46.370X - 0.6728（r = 0.9999，n = 3）。根皮苷的平均回收率为99.40%，相对标准偏差（RSD）为0.67%。
该方法用于测定根皮苷含量简便、快速、准确。
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