Goldberg R L, Rubin A S
Research Department, Pharmaceuticals Division, CIBA-GEIGY Corporation, Summit, NJ 07901.
J Rheumatol. 1989 Jan;16(1):92-6.
Serum concentrations of hyaluronate may provide a clinically relevant, quantitative marker of disease in patients with active rheumatoid arthritis (RA). We studied the utility of serum hyaluronate in an animal model, with features reminiscent of human RA, in which LEW/N female rats were made arthritic by intraperitoneal injection of sonicated Lactobacillus casei. When serum hyaluronate was measured by an inhibition ELISA, a dose dependent correlation was found between the amount of L. casei injected and both joint score and serum hyaluronate in the chronic phase of the disease. A linear correlation between the chronic phase joint score and serum hyaluronate was observed (r = 0.69, p less than 0.001). Two orally administered compounds, flurbiprofen (20 mg/kg) and methotrexate (0.125 mg/kg), were effective in decreasing both variables. Thus, serum hyaluronate may have utility in evaluating the therapeutic efficacy of antirheumatic/antiinflammatory agents in vivo in the chronic phase of RA-like diseases.
透明质酸盐的血清浓度可能为活动性类风湿关节炎（RA）患者提供一种具有临床相关性的疾病定量标志物。我们在一种具有类似人类RA特征的动物模型中研究了血清透明质酸盐的效用，该模型中通过腹腔注射超声处理的干酪乳杆菌使LEW/N雌性大鼠患关节炎。当通过抑制ELISA法测量血清透明质酸盐时，在疾病的慢性期，发现注射的干酪乳杆菌数量与关节评分及血清透明质酸盐之间存在剂量依赖性相关性。观察到慢性期关节评分与血清透明质酸盐之间存在线性相关性（r = 0.69，p小于0.001）。两种口服化合物，氟比洛芬（20 mg/kg）和甲氨蝶呤（0.125 mg/kg），在降低这两个变量方面均有效。因此，血清透明质酸盐可能有助于在类风湿关节炎样疾病的慢性期体内评估抗风湿/抗炎药物的治疗效果。
