Wang Hui, Ji Bei, Wang Junfeng, Guo Fajin, Zhou Wenjun, Gao Lili, Liu Tian Zhong
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, People's Republic of China.
Bioprocess Biosyst Eng. 2014 Dec;37(12):2607-13. doi: 10.1007/s00449-014-1238-x. Epub 2014 Jun 28.
Filamentous oleaginous microalgae Tribonema minus have advantages in relatively easy harvesting and grazers resistance in mass cultivation due to its filaments in previous study. To evaluate whether the genus Tribonema is a valuable candidate for use in biofuel production, the morphology, growth, biochemical composition and fatty acid profile of six filamentous microalgae strains Tribonema sp. were investigated. All the strains are unbranched filament in single row of elongated cylinder, attaining 0.5-3 mm in length. The growth rates of tested strains were 0.35-0.42 g L(-1) d(-1). Generally, for all strains, decrease in protein content was followed by a slight increase in lipid and significant increase in carbohydrate in early phase, afterwards, lipid increased constantly inversely to decrease in carbohydrate content. After 15-day cultivation, total lipid contents of tested strains ranged from 38-61 %, of which TAG were the majority and palmitic acid (C16:0) and palmitoleic acid (C16:1) were the dominant components. The study confirmed that the genus Tribonema is the potential for biodiesel and bioethanol production upon culture time.
丝状产油微藻纤细三角藻(Tribonema minus)由于其丝状结构,在以往研究中显示出在大规模培养中收获相对容易且抗食草动物的优势。为了评估三角藻属是否是用于生物燃料生产的有价值候选者,对六种丝状微藻菌株纤细三角藻(Tribonema sp.)的形态、生长、生化组成和脂肪酸谱进行了研究。所有菌株均为单列细长圆柱体排列的无分支丝状体,长度达0.5 - 3毫米。受试菌株的生长速率为0.35 - 0.42克/升·天。一般来说,对于所有菌株,在早期蛋白质含量下降后,脂质略有增加,碳水化合物显著增加,之后,脂质持续增加,与碳水化合物含量下降呈反比。培养15天后,受试菌株的总脂质含量在38% - 61%之间,其中三酰甘油占多数,棕榈酸(C16:0)和棕榈油酸(C16:1)是主要成分。该研究证实,随着培养时间的推移,三角藻属具有生产生物柴油和生物乙醇的潜力。