增强丝状产油微藻 Tribonema 油脂产量的策略研究。
Strategy study on enhancing lipid productivity of filamentous oleaginous microalgae Tribonema.
机构信息
CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
出版信息
Bioresour Technol. 2016 Oct;218:161-6. doi: 10.1016/j.biortech.2016.06.083. Epub 2016 Jun 23.
Filamentous microalgae Tribonema is promising for biodiesel production in terms of high lipid content, easy harvesting and good contamination tolerance. As high lipid productivity is a key basis, several methods including different culture medium, phosphorus, iron and CO2 inductions were tested to enhance lipid productivity in Tribonema minus. T. minus subjected to BG11-freshwater medium achieved a favorable lipid productivity of 216.18mgL(-1)d(-1), much higher than those in other culture medium (BG11-S, Kuhl, f/2, f, 2f, BBM, ASW and ES). Secondly, the optimal phosphorus, iron and CO2 concentrations for high lipid productivity of T. minus were obtained, respectively. Finally, the cells of T. minus grown in optimized BG11 medium containing 80mgL(-1) KH2PO4 and 6mgL(-1) FeC6H5O7·NH4OH with 2% CO2 achieved a lipid productivity of 384.67mgL(-1)d(-1), representing a 1.56-fold increase from the control. Therefore, the strategy to enhance lipid productivity of T. minus was successfully proposed.
丝状微藻 Tribonema 具有脂质含量高、易于收获和良好的抗污染能力等特点，有望用于生物柴油生产。高脂质生产力是关键基础，因此测试了几种方法，包括不同的培养基、磷、铁和 CO2 诱导，以提高 Tribonema minus 的脂质生产力。T.minus 在 BG11-淡水培养基中实现了 216.18mgL(-1)d(-1)的良好脂质生产力，明显高于其他培养基（BG11-S、Kuhl、f/2、f、2f、BBM、ASW 和 ES）。其次，确定了 T.minus 获得高脂质生产力的最佳磷、铁和 CO2 浓度。最后，在优化的含有 80mgL(-1) KH2PO4 和 6mgL(-1) FeC6H5O7·NH4OH 的 BG11 培养基中，T.minus 的细胞在 2% CO2 下生长，实现了 384.67mgL(-1)d(-1)的脂质生产力，比对照提高了 1.56 倍。因此，成功提出了提高 T.minus 脂质生产力的策略。
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