Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
BMC Complement Altern Med. 2014 Jun 28;14:207. doi: 10.1186/1472-6882-14-207.
Recent studies have revealed that destruxins (Dtx) have potent cytotoxic activities on individual cancer cells, however, data on oral cancer cells especial human are absent.
Destruxin B (DB) was isolated and used to evaluate the selective cytotoxicity with human oral cancer cell lines, GNM (Neck metastasis of gingival carcinoma) and TSCCa (Tongue squamous cell carcinoma) cells, and normal gingival fibroblasts (GF) were also included as controls. Cells were tested with different concentrations of DB for 24, 48, and 72 h by MTT assay. Moreover, the mechanism of cytotoxicity was investigated using caspase-3 Immunofluorescence, annexin V/PI staining, and the expression of caspase-3, Bax, and Bcl-2 by western blotting after treated with different concentrations of DB for 72 h as parameters for apoptosis analyses.
The results show that DB exhibited significant (p < 0.01) and selective time- and dose-dependent inhibitory effects on GNM and TSCCa cells viability but not on GF cells. The data suggested that DB is capable to induce tumor specific growth inhibition in oral GNM and TSCCa cancer cells via Bax/Bcl-2-mediated intrinsic mitochondrial apoptotic pathway in time- and dose-dependent manners.
This is the first report on the anti-proliferation effect of DB in oral cancer cells. The results reported here may offer further evidences to the development of DB as a potential complementary chemotherapeutic target for oral cancer complications.
最近的研究表明， destruxins（Dtx）对个别癌细胞具有强烈的细胞毒性作用，但缺乏关于口腔癌细胞特别是人类口腔癌细胞的资料。
分离 destruxin B（DB）并用于评估其对人口腔癌细胞系 GNM（牙龈癌颈部转移）和 TSCCa（舌鳞状细胞癌）的选择性细胞毒性，同时还包括正常牙龈成纤维细胞（GF）作为对照。通过 MTT 法，用不同浓度的 DB 处理细胞 24、48 和 72 h。此外，通过 caspase-3 免疫荧光、膜联蛋白 V/PI 染色以及用不同浓度的 DB 处理 72 h 后 caspase-3、Bax 和 Bcl-2 的表达western 印迹，研究细胞毒性的机制，作为凋亡分析的参数。
结果表明，DB 对 GNM 和 TSCCa 细胞的活力表现出显著的（p<0.01）、选择性的、时间和剂量依赖性抑制作用，但对 GF 细胞没有作用。数据表明，DB 通过 Bax/Bcl-2 介导的内在线粒体凋亡途径，以时间和剂量依赖的方式，能够诱导口腔 GNM 和 TSCCa 癌细胞的肿瘤特异性生长抑制。
这是关于 DB 在口腔癌细胞中增殖抑制作用的首次报道。这里报道的结果可能为 DB 作为口腔癌并发症的潜在辅助化学治疗靶标的发展提供进一步的证据。
