Long Youming, Zheng Yangbo, Shan Fulan, Chen Mengyu, Fan Yongxiang, Zhang Bin, Gao Cong, Gao Qingchun, Yang Ning
Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
J Neuroimmunol. 2014 Aug 15;273(1-2):103-10. doi: 10.1016/j.jneuroim.2014.06.003. Epub 2014 Jun 12.
To develop a cell-based assay (CBA) to detect aquaporin 1 (AQP1) antibodies and determine sensitivity/specificity in patients with neuromyelitis optica (NMO) spectrum disorders.
A HEK-293T transfected cell model expressing AQP1 was established and detected to be serum AQP1 antibodies.
AQP1 antibodies were present in 73/98 (74.5%) AQP4 antibody-positive patients. Some AQP4 antibody-negative patients were also AQP1 antibody-positive. Test sensitivity was 74.5% in 98 AQP4 antibody-positive patients. Test specificity was 79.6% in 67 multiple sclerosis (MS) patients and 31 controls.
A sensitive and simple CBA was developed to detect serum AQP1 antibodies. AQP1 antibodies were mainly present in NMO and its high-risk syndrome, but also in some MS patients.
开发一种基于细胞的检测方法（CBA）以检测水通道蛋白1（AQP1）抗体，并确定视神经脊髓炎谱系障碍（NMO）患者的敏感性/特异性。
建立表达AQP1的HEK-293T转染细胞模型，并检测血清中的AQP1抗体。
98例AQP4抗体阳性患者中有73例（74.5%）存在AQP1抗体。一些AQP4抗体阴性患者也是AQP1抗体阳性。在98例AQP4抗体阳性患者中检测敏感性为74.5%。在67例多发性硬化症（MS）患者和31例对照中检测特异性为79.6%。
开发了一种灵敏且简单的CBA来检测血清AQP1抗体。AQP1抗体主要存在于NMO及其高危综合征中，但也存在于一些MS患者中。
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