用于检测视神经脊髓炎谱系障碍中抗水通道蛋白1抗体的细胞检测方法的开发。

Development of a cell-based assay for the detection of anti-aquaporin 1 antibodies in neuromyelitis optica spectrum disorders.

作者信息

Long Youming, Zheng Yangbo, Shan Fulan, Chen Mengyu, Fan Yongxiang, Zhang Bin, Gao Cong, Gao Qingchun, Yang Ning

机构信息

Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.

出版信息

J Neuroimmunol. 2014 Aug 15;273(1-2):103-10. doi: 10.1016/j.jneuroim.2014.06.003. Epub 2014 Jun 12.

DOI:10.1016/j.jneuroim.2014.06.003

PMID:24972850

Abstract

OBJECTIVE

To develop a cell-based assay (CBA) to detect aquaporin 1 (AQP1) antibodies and determine sensitivity/specificity in patients with neuromyelitis optica (NMO) spectrum disorders.

METHODS

A HEK-293T transfected cell model expressing AQP1 was established and detected to be serum AQP1 antibodies.

RESULTS

AQP1 antibodies were present in 73/98 (74.5%) AQP4 antibody-positive patients. Some AQP4 antibody-negative patients were also AQP1 antibody-positive. Test sensitivity was 74.5% in 98 AQP4 antibody-positive patients. Test specificity was 79.6% in 67 multiple sclerosis (MS) patients and 31 controls.

CONCLUSION

A sensitive and simple CBA was developed to detect serum AQP1 antibodies. AQP1 antibodies were mainly present in NMO and its high-risk syndrome, but also in some MS patients.

摘要