Barbas J A, Rubio N, Pedroso E, Pongs O, Ferrús A
Instituto Cajal, CSIC, Madrid, Spain.
Brain Res Mol Brain Res. 1989 Mar;5(2):171-6. doi: 10.1016/0169-328x(89)90008-9.
Shaker is a complex locus (ShC) in Drosophila that encodes components of the K+ channel responsible for the IA current. We have raised antibodies against synthetic peptides of selected sequences from the Sh products. One of the antisera identifies a 71 kDa protein band in immunoblots from Drosophila neural membrane proteins. We demonstrate that this protein is encoded within the viable (V) region of the ShC since deletions and breakpoints in this part of the complex eliminate this band from the immunoblots. Certain Sh mutations abolish the production of this product while other do not seem to interfere with it. The same antiserum identifies bands of different apparent molecular weight (Mr) in membrane extracts of nervous systems of a variety of organisms including vertebrates.
震荡器是果蝇中的一个复杂基因座（ShC），它编码负责IA电流的钾离子通道的组成部分。我们针对Sh产物中选定序列的合成肽制备了抗体。其中一种抗血清在果蝇神经膜蛋白的免疫印迹中识别出一条71 kDa的蛋白条带。我们证明，这种蛋白是由ShC的存活（V）区域编码的，因为该复合体这一部分的缺失和断点会从免疫印迹中消除这条带。某些Sh突变会消除该产物的产生，而其他突变似乎不会对其产生干扰。同一种抗血清在包括脊椎动物在内的多种生物体的神经系统膜提取物中识别出不同表观分子量（Mr）的条带。
