Papazian D M, Schwarz T L, Tempel B L, Jan Y N, Jan L Y
Science. 1987 Aug 14;237(4816):749-53. doi: 10.1126/science.2441470.
On the basis of electrophysiological analysis of Shaker mutants, the Shaker locus of Drosophila melanogaster has been proposed to encode a structural component of a voltage-dependent potassium channel, the A channel. Unlike sodium channels, acetylcholine receptors, and calcium channels, K+ channels have not been purified biochemically. To facilitate biochemical studies of a K+ channel, genomic DNA from the Shaker locus has been cloned. Rearrangements in five Shaker mutants have been mapped to a 60-kilobase segment of the genome. Four complementary DNA clones have been analyzed. These clones indicate that the Shaker gene contains multiple exons distributed over at least 65 kilobases of genomic DNA in the region where the mutations mapped. Furthermore, the gene may produce several classes of alternatively spliced transcripts. Two of the complementary DNA clones have been sequenced and their sequences support the hypothesis that Shaker encodes a component of a K+ channel.
基于对“震颤者”突变体的电生理分析，有人提出果蝇的“震颤者”基因座编码一种电压依赖性钾通道（A通道）的结构成分。与钠通道、乙酰胆碱受体和钙通道不同，钾离子通道尚未通过生化方法纯化。为便于对钾离子通道进行生化研究，已克隆了来自“震颤者”基因座的基因组DNA。五个“震颤者”突变体中的重排已定位到基因组的一个60千碱基片段上。已分析了四个互补DNA克隆。这些克隆表明，“震颤者”基因包含多个外显子，分布在突变定位区域至少65千碱基的基因组DNA上。此外，该基因可能产生几类可变剪接转录本。已对两个互补DNA克隆进行了测序，它们的序列支持“震颤者”编码钾离子通道成分的假说。
