Li Haiyin, Zhang Xiqi, Zhang Xiaoyong, Yang Bin, Wei Yen
College of Chemistry and Pharmaceutical Sciences, Qingdao Agriculture University, Qingdao 266109, PR China.
Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084, PR China.
Colloids Surf B Biointerfaces. 2014 Sep 1;121:347-53. doi: 10.1016/j.colsurfb.2014.06.015. Epub 2014 Jun 12.
Self-assembly of polymeric materials to form nanoparticles is a particularly promising strategy for various biomedical applications, however, these self-assembling systems often encounter the critical micelle concentration (CMC) issue, as the nanoparticles is usually unstable at low concentration. Therefore, stable cross-linked fluorescent polymeric nanoparticles (FPNs) were covalently constructed from an aggregation induced emission (AIE) dye, itaconic anhydride, poly(ethylene glycol) monomethyl ether methacylate and polyethylenimine. These obtained PhE-ITA-20%(80%) FPNs were fully characterized by a series of techniques including (1)H NMR spectra, UV-vis absorption spectra, fluorescence spectra, FT-IR spectra, transmission electron microscopy, gel permeation chromatography, and dynamic light scattering. Such FPNs emitted intense fluorescence due to the introduction of aggregation induced emission dye. More importantly, the FPNs were found extremely stable in physiological solution even below the CMC owing to their cross-linked architectures. Biocompatibility evaluation and cell uptake behavior of the FPNs were further investigated to explore their potential biomedical applications, the demonstrated excellent biocompatibility made them promising for cell imaging.
聚合物材料自组装形成纳米颗粒是一种在各种生物医学应用中特别有前景的策略，然而，这些自组装系统常常遇到临界胶束浓度（CMC）问题，因为纳米颗粒在低浓度时通常不稳定。因此，通过聚集诱导发光（AIE）染料、衣康酸酐、聚乙二醇单甲醚甲基丙烯酸酯和聚乙烯亚胺共价构建了稳定的交联荧光聚合物纳米颗粒（FPNs）。通过一系列技术对所获得的PhE-ITA-20%（80%）FPNs进行了全面表征，包括核磁共振氢谱、紫外可见吸收光谱、荧光光谱、傅里叶变换红外光谱、透射电子显微镜、凝胶渗透色谱和动态光散射。由于引入了聚集诱导发光染料，此类FPNs发出强烈荧光。更重要的是，由于其交联结构，发现FPNs在生理溶液中即使低于CMC也极其稳定。进一步研究了FPNs的生物相容性评估和细胞摄取行为以探索其潜在的生物医学应用，所证明的优异生物相容性使其在细胞成像方面具有前景。
