School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
Chem Biol Interact. 2014 Sep 5;220:75-83. doi: 10.1016/j.cbi.2014.06.018. Epub 2014 Jun 26.
The study was to characterize the polyphenolic composition, antioxidant properties, and hepatoprotective effects of a polyphenols-enriched extract (HMTP) from Huangshan Maofeng green tea. HPLC analysis showed that three predominantly polyphenolic compounds present in HMTP were epigallocatechin (271.2 μg/mg extract), rutin (239.3 μg/mg) and epicatechin (89.3 μg/mg). HMTP was shown to exhibit strong scavenging activities against DPPH, O2(-), and OH, and ferric-reducing antioxidant power in vitro. Administration of HMTP at 200, 400 and 800 mg/kg bw in mice prior to CCl4 injury significantly decreased the CCl4-induced elevation of serum ALT, AST and ALP activities, and prevented an increase in hepatic MDA levels (p<0.05). Mice with HMTP pretreatment displayed a better profile of hepatosomatic index and the improved GSH-Px and SOD activities in the liver, relative to CCl4-intoxicated mice. Liver pathological observation also confirmed the protection on CCl4-caused histological alteration, suggesting that HMTP has potential to be explored as valuable hepatoprotective function food.
该研究旨在表征黄山毛峰绿茶多酚富集提取物（HMTP）的多酚组成、抗氧化特性和肝保护作用。HPLC 分析表明，HMTP 中存在三种主要的多酚化合物，分别为表没食子儿茶素（271.2 μg/mg 提取物）、芦丁（239.3 μg/mg）和表儿茶素（89.3 μg/mg）。HMTP 表现出对 DPPH、O2(-) 和 OH 的强烈清除活性，以及体外铁还原抗氧化能力。在 CCl4 损伤前，以 200、400 和 800 mg/kg bw 的剂量给小鼠灌胃 HMTP，可显著降低 CCl4 引起的血清 ALT、AST 和 ALP 活性升高，并防止肝 MDA 水平升高（p<0.05）。与 CCl4 中毒的小鼠相比，用 HMTP 预处理的小鼠具有更好的肝体比指数和改善的肝 GSH-Px 和 SOD 活性。肝组织学观察也证实了对 CCl4 引起的组织学改变的保护作用，表明 HMTP 具有作为有价值的肝保护功能食品进行开发的潜力。
