Fissolo Nicolás, Montalban Xavier, Comabella Manuel
Department of Neurology-Neuroimmunology, Centre d'Esclerosi Múltiple de Catalunya, Cemcat, Institut de Recerca Vall d'Hebron, Hospital Universitari Vall d´Hebron (HUVH), Barcelona, Spain,
Methods Mol Biol. 2016;1304:39-50. doi: 10.1007/7651_2014_87.
Multiple sclerosis (MS) is the most common inflammatory, demyelinating, and neurodegenerative disorder of the central nervous system (CNS) in humans. Although the etiology of MS remains unknown, several lines of evidence support the notion that autoimmunity against components of the myelin sheath plays a major role in susceptibility to and development of the disease. At present, there are no approved MS therapies aimed specifically toward downregulating antigen-specific autoreactive immune cells. One antigen-specific approach that appears promising for the treatment of MS is DNA vaccination. This technique has demonstrated efficacy in clinical trials while maintaining safety.Here, we describe the generation of DNA vaccines containing immunologically relevant antigens of MS. Moreover, we present a detailed protocol for the prophylactic and therapeutic administration of DNA vaccines via intramuscular injection targeting on the development of experimental autoimmune encephalomyelitis (EAE), an animal model resembling MS.
多发性硬化症（MS）是人类中枢神经系统（CNS）最常见的炎性、脱髓鞘性和神经退行性疾病。尽管MS的病因尚不清楚，但有几条证据支持这样的观点，即针对髓鞘成分的自身免疫在该疾病的易感性和发展中起主要作用。目前，尚无专门针对下调抗原特异性自身反应性免疫细胞的获批MS疗法。一种对MS治疗似乎有前景的抗原特异性方法是DNA疫苗接种。该技术在临床试验中已证明有效且安全性良好。在此，我们描述了包含MS免疫相关抗原的DNA疫苗的制备。此外，我们还提供了一份详细方案，用于通过肌肉注射预防性和治疗性给药DNA疫苗，以针对实验性自身免疫性脑脊髓炎（EAE）的发展，EAE是一种类似于MS的动物模型。
