Ross Stephen T, Allen John R, Davidson Michael W
Nikon Instruments, Inc., Melville, New York, USA.
National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, USA.
Methods Cell Biol. 2014;123:19-34. doi: 10.1016/B978-0-12-420138-5.00002-1.
For nearly a century, examination of biological phenomena on a microscopic level has depended on carefully calibrated optical systems, with the objective lens being regarded as the critical determinant of image quality. In this modern day, a wide variety of high-quality objectives exist, many with highly specialized functions and all requiring at least a certain base level of knowledge on the part of the imager in order to realize their full potential. A good working knowledge of objective construction, proper use, specialized applications, and care goes a long way toward obtaining quality results. Presented here is a practical guide to choosing, using, and maintaining the proper objective for a given biological imaging application.
近一个世纪以来，在微观层面上对生物现象的观察一直依赖于经过精心校准的光学系统，其中物镜被视为图像质量的关键决定因素。在当今时代，存在各种各样的高质量物镜，许多具有高度专业化的功能，并且成像者都需要至少具备一定的基础知识，才能充分发挥其潜力。对物镜的构造、正确使用、特殊应用和保养有良好的实际了解，对于获得高质量结果大有帮助。本文介绍了一份实用指南，用于为特定的生物成像应用选择、使用和维护合适的物镜。
