University of Grenoble 1/CNRS, LIPhy UMR 5588, Grenoble, France.
Opt Lett. 2013 Jul 15;38(14):2401-3. doi: 10.1364/OL.38.002401.
We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples.
我们描述了在通过单个活细胞聚焦时，像差对荧光波动显微镜（FFM）的影响。FFM 测量是在水性荧光溶液中进行的，事实证明这是一种非常灵敏的工具，可以评估细胞引入的像差。我们展示了一种自适应光学（AO）系统，可以消除 FFM 测量中的像差相关偏差。我们的数据表明，AO 不仅在对组织深处进行成像时有用，而且在通过单个细胞层进行 FFM 测量时也有用。这项工作为将 FFM 应用于复杂的三维多细胞样本铺平了道路。
