Romeo Stefania, Trupiano Dalila, Ariani Andrea, Renzone Giovanni, Scippa Gabriella S, Scaloni Andrea, Sebastiani Luca
BioLabs, Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127 Pisa, Italy.
Dipartimento di Bioscienze e Territorio, University of Molise, 86090 Pesche, IS, Italy.
J Plant Physiol. 2014 Jul 15;171(12):1054-63. doi: 10.1016/j.jplph.2014.03.016. Epub 2014 Apr 18.
Contamination of soil and water by heavy metals has become a widespread problem; environmental pollution by high zinc (Zn) concentration occurs frequently. Although poplar (Populus spp.) has been identified as suitable for phytoremediation approaches, its response to high Zn concentrations are still not clearly understood. For this reason, we investigated the effects of Zn in Populus×euramericana clone I-214 roots by proteomic analysis. Comparative experiments were conducted on rooted woody cuttings grown in nutrient solutions containing 1mM (treatment) or 1μM (control) Zn concentrations. A gel-based proteomic approach coupled with morphological and chemical analysis was used to identify differentially represented proteins in treated roots and to investigate the effect of Zn treatment on the poplar root system. Data shows that Zn was accumulated preferentially in roots, that the antioxidant system, the carbohydrate/energy and amino acid metabolisms were the main pathways modulated by Zn excess, and that mitochondria and vacuoles were the cellular organelles predominately affected by Zn stress. A coordination between cell death and proliferation/growth seems to occur under this condition to counteract the Zn-induced damage.
土壤和水体受到重金属污染已成为一个普遍存在的问题；高锌（Zn）浓度造成的环境污染屡见不鲜。尽管杨树（Populus spp.）已被确定适用于植物修复方法，但其对高锌浓度的反应仍未完全明晰。因此，我们通过蛋白质组学分析研究了锌对欧美杨无性系I-214根系的影响。对在含有1mM（处理组）或1μM（对照组）锌浓度的营养液中生长的带根木质插条进行了对比实验。采用基于凝胶的蛋白质组学方法，结合形态学和化学分析，以鉴定处理过的根系中差异表达的蛋白质，并研究锌处理对杨树根系系统的影响。数据表明，锌优先在根系中积累，抗氧化系统、碳水化合物/能量和氨基酸代谢是受锌过量调节的主要途径，线粒体和液泡是受锌胁迫影响最主要的细胞器。在这种情况下，细胞死亡与增殖/生长之间似乎存在一种协调作用，以抵消锌诱导的损伤。
