Han Miao-Miao, Wang Wen-Fei, Liu Ming-Yao, Li De-Shan, Zhou Bing, Yu Yin-Hang, Ren Gui-Ping
Yao Xue Xue Bao. 2014 Apr;49(4):470-5.
Fibroblast growth factor-21 (FGF-21) is an important metabolism regulator, however, whether FGF-21 has effects on cardiovascular remains unclear. In this study, H2O2-induced injury in H9c2 cells was used as a cell model, the anti-apoptosis potential and mechanism of FGF-21 against oxidative injury were evaluated by MTT assay, flow cytometry assay and real-time PCR. The results showed that FGF-21 could increase the cell survival of H2O2-induced injury in H9c2 cells and prevent H9c2 cells from oxidative stress-induced apoptosis. Furthermore, FGF-21 can elevate SOD activity and regulate Bcl-2/Bax expression in H9c2 cells. The results suggest that FGF-21 have protective effect against the H2O2-induced apoptosis in H9c2 cells.
成纤维细胞生长因子21（FGF - 21）是一种重要的代谢调节因子，然而，FGF - 21是否对心血管有影响仍不清楚。在本研究中，以H2O2诱导H9c2细胞损伤作为细胞模型，通过MTT法、流式细胞术检测和实时定量PCR评估FGF - 21抗凋亡的潜能及其抗氧化损伤的机制。结果显示，FGF - 21可提高H2O2诱导损伤的H9c2细胞的存活率，并防止H9c2细胞发生氧化应激诱导的凋亡。此外，FGF - 21可提高H9c2细胞的超氧化物歧化酶（SOD）活性，并调节Bcl - 2/Bax的表达。结果表明，FGF - 21对H2O2诱导的H9c2细胞凋亡具有保护作用。
