College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Department of Pharmacy, People's Hospital of Gaotang County, Shandong 252800, China.
Biomed Pharmacother. 2017 Oct;94:72-78. doi: 10.1016/j.biopha.2017.07.096. Epub 2017 Aug 16.
The preliminary studies have shown that Dendrobium officinale possessed therapeutic effects on hypertension and atherosclerosis. Studies also reported that Dendrobium officinale polysaccharides showed antioxidant capabilities. However, little is known about its effects on myocardial cells under oxidative stress. The present study was designed to study the protective effect of Dendrobium officinale polysaccharides against HO-induced oxidative stress in H9c2 cells. MTT assay was carried out to determine the cell viability of H9c2 cells when pretreated with Dendrobium officinale polysaccharides. Fluorescent microscopy measurements were performed for evaluating the apoptosis in H9c2 cells. Furthermore, effects of Dendrobium officinale polysaccharides on the activities of antioxidative indicators (malondialdehyde, superoxide dismutase), reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP) levels were analyzed. Dendrobium officinale polysaccharides attenuated HO-induced cell death, as determined by the MTT assay. Dendrobium officinale polysaccharides decreased malondialdehyde levels, increased superoxide dismutase activities, and inhibited the generation of intracellular ROS. Moreover, pretreatment with Dendrobium officinale polysaccharides also inhibited apoptosis and increased the MMP levels in H9c2 cells. These results suggested the protective effects of Dendrobium officinale polysaccharides against HO-induced injury in H9c2 cells. The results also indicated the anti-oxidative capability of Dendrobium officinale polysaccharides.
初步研究表明，铁皮石斛对高血压和动脉粥样硬化具有治疗作用。研究还报道铁皮石斛多糖具有抗氧化能力。然而，关于其在氧化应激下对心肌细胞的影响知之甚少。本研究旨在研究铁皮石斛多糖对 H9c2 细胞中 HO 诱导的氧化应激的保护作用。MTT 法测定铁皮石斛多糖预处理对 H9c2 细胞活力的影响。荧光显微镜测量用于评估 H9c2 细胞的凋亡。此外，还分析了铁皮石斛多糖对抗氧化指标（丙二醛、超氧化物歧化酶）活性、活性氧（ROS）产生和线粒体膜电位（MMP）水平的影响。MTT 法测定铁皮石斛多糖减轻了 HO 诱导的细胞死亡。铁皮石斛多糖降低丙二醛水平，增加超氧化物歧化酶活性，抑制细胞内 ROS 的产生。此外，铁皮石斛多糖预处理还抑制 H9c2 细胞的凋亡并增加 MMP 水平。这些结果表明铁皮石斛多糖对 H9c2 细胞中 HO 诱导损伤具有保护作用。结果还表明铁皮石斛多糖具有抗氧化能力。
