Guo Wenjie, Yang Min, Liang Xufang, Lv Liyuan, Xiao Tiaoyi, Tian Changxu
a College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , Hubei , China .
b Hubei Collaborative Innovation Center for Freshwater Aquaculture , Wuhan , Hubei , China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1094-5. doi: 10.3109/19401736.2014.930838. Epub 2014 Jun 30.
In this study, we determined the complete mitochondrial DNA sequence of the hybrid of Siniperca chuatsi (♀) × Siniperca scherzeri (♂). The total length of the hybrid mitogenome is 16,497 bp, with the base composition of 28.61% A, 29.16% C, 16.23% G, and 26.00% T. It contains 2 rRNA genes, 13 protein-coding genes, 22 tRNA genes, and 1 control region. The composition and order of these genes are identical to most of other vertebrates. All the protein-coding genes start with ATG, except for COX1 with the initiation codon GTG. Three types of stop codons are used by the coding genes, including complete stop codons (TAA and TAG) and an incomplete stop codon (T). Three conserved domains were observed in the control region. The complete mitogenome of the hybrid of S. chuatsi (♀) × S. scherzeri (♂) could contribute to a better solution of the study in mitochondrial inheritance mechanism.
在本研究中,我们测定了鳜(♀)×斑鳜(♂)杂交种的完整线粒体DNA序列。杂交种线粒体基因组全长16497bp,碱基组成为28.61%A、29.16%C、16.23%G和26.00%T。它包含2个rRNA基因、13个蛋白质编码基因、22个tRNA基因和1个控制区。这些基因的组成和顺序与大多数其他脊椎动物相同。除COX1的起始密码子为GTG外,所有蛋白质编码基因均以ATG起始。编码基因使用三种类型的终止密码子,包括完整终止密码子(TAA和TAG)和不完整终止密码子(T)。在控制区观察到三个保守结构域。鳜(♀)×斑鳜(♂)杂交种的完整线粒体基因组有助于更好地解决线粒体遗传机制的研究问题。