Molina Nathalia, Morandi Ana Carolina, Bolin Anaysa Paola, Otton Rosemari
Postgraduate Program, Health Sciences, CBS, Universidade Cruzeiro do Sul, São Paulo, SP, Brazil.
Postgraduate Program, Health Sciences, CBS, Universidade Cruzeiro do Sul, São Paulo, SP, Brazil.
Int Immunopharmacol. 2014 Sep;22(1):41-50. doi: 10.1016/j.intimp.2014.06.026. Epub 2014 Jun 24.
The aim of this study was to evaluate the effects of FUCO alone or combined with vitamin C on different features of lymphocyte function related to ROS/RNS (reactive oxygen/nitrogen species) production. For this purpose we have evaluated the cytotoxicity of increasing concentrations of FUCO and vitamin C, the proliferative capacity of stimulated T- and B-lymphocytes, superoxide anion radicals (O(2)), hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) production, antioxidant enzyme activities and the indexes of oxidative damage in proteins (carbonyl and thiol content). We have also evaluated the release of inflammatory cytokines and glucose-6-phosphate dehydrogenase (G6PDH) activity. Healthy human lymphocytes were acutely treated in vitro with FUCO (2 μM) with or without vitamin C (100 μM). Results revealed that human lymphocytes treated with FUCO at 2μM did not present any significant alteration in the proliferation of T- and B-lymphocytes at both resting and stimulated conditions. Moreover, FUCO used at low concentrations showed more pro-oxidant than antioxidant effects, which were recognized by the increased H(2)O(2) and increased NO production. Anti-inflammatory activity of FUCO was confirmed by significantly increased IL-10 and decreased TNF-α production. Vitamin C increased T-lymphocyte proliferation, whereas vitamin C plus FUCO promoted a reduction in the proliferation rate of these cells. All groups decreased pro-inflammatory cytokine TNF-α and increased anti-inflammatory IL-10 production although only vitamin C decreased IFN-γ either alone or when combined with FUCO. Overall, the combination of the antioxidants had more antioxidant and anti-inflammatory effects than when they were applied alone.
本研究的目的是评估岩藻聚糖单独使用或与维生素C联合使用对与活性氧/氮物种（ROS/RNS）产生相关的淋巴细胞功能不同特征的影响。为此，我们评估了岩藻聚糖和维生素C浓度增加时的细胞毒性、刺激的T淋巴细胞和B淋巴细胞的增殖能力、超氧阴离子自由基（O₂）、过氧化氢（H₂O₂）和一氧化氮（NO）的产生、抗氧化酶活性以及蛋白质氧化损伤指标（羰基和巯基含量）。我们还评估了炎性细胞因子的释放和葡萄糖-6-磷酸脱氢酶（G6PDH）活性。健康人淋巴细胞在体外分别用岩藻聚糖（2μM）单独处理或与维生素C（100μM）联合处理。结果显示，用2μM岩藻聚糖处理的人淋巴细胞在静息和刺激条件下，T淋巴细胞和B淋巴细胞的增殖均未出现任何显著变化。此外，低浓度使用的岩藻聚糖显示出更多的促氧化而非抗氧化作用，这表现为H₂O₂增加和NO产生增加。岩藻聚糖的抗炎活性通过IL-10显著增加和TNF-α产生减少得到证实。维生素C增加T淋巴细胞增殖，而维生素C加岩藻聚糖则促进这些细胞增殖率降低。所有组均降低促炎细胞因子TNF-α并增加抗炎性IL-
