Wirth K A, Kow K, Salute M E, Bacon N J, Milner R J
Department of Clinical Sciences, University of Florida, Gainesville, FL, USA.
Vet Comp Oncol. 2016 Sep;14(3):281-94. doi: 10.1111/vco.12100. Epub 2014 Jun 29.
Yunnan Baiyao is a Chinese herbal medicine that has been utilized for its anti-inflammatory, haemostatic, wound healing and pain relieving properties in people. It has been utilized in the veterinary profession to control bleeding in dogs with hemangiosarcoma (HSA) and has been anecdotally reported to prolong survival times in dogs with this neoplasm. This study evaluated the in vitro activity of Yunnan Baiyao against three canine HSA cell lines after treatment with increasing concentrations of Yunnan Baiyao (50, 100, 200, 400, 600 and 800 µg mL(-1) ) at 24, 48 and 72 h. Mean half maximum inhibitory concentration (IC50 ) at 72 h for DEN, Fitz, SB was 369.9, 275.9 and 325.3 µg mL(-1) , respectively. Caspase-3/7 activity increased in correlation with the IC50 in each cell line which was confirmed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, APO-BRDU Kit; BD Biosciences, San Jose, CA, USA) assay. VEGF in cell supernatant was also quantified. Overall, the study found that Yunnan Baiyao causes dose and time dependent HSA cell death through initiation of caspase-mediated apoptosis, which supports future studies involving Yunnan Baiyao.
云南白药是一种中草药，因其具有抗炎、止血、促进伤口愈合和止痛特性而被人们使用。它已被应用于兽医领域，用于控制患有血管肉瘤（HSA）的犬类出血，并且有传闻称它能延长患有这种肿瘤的犬类的存活时间。本研究评估了在24、48和72小时用递增浓度的云南白药（50、100、200、400、600和800μg mL⁻¹）处理后，云南白药对三种犬类HSA细胞系的体外活性。DEN、Fitz、SB在72小时的平均半数最大抑制浓度（IC50）分别为369.9、275.9和325.3μg mL⁻¹。半胱天冬酶-3/7活性在每个细胞系中与IC50呈正相关，这通过末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL，APO-BRDU试剂盒；BD生物科学公司，美国加利福尼亚州圣何塞）检测得到证实。还对细胞上清液中的血管内皮生长因子（VEGF）进行了定量。总体而言，该研究发现云南白药通过启动半胱天冬酶介导的凋亡导致剂量和时间依赖性的HSA细胞死亡，这为涉及云南白药的未来研究提供了支持。
