Laboratory Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China; Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Department of Neurology, The First Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Urology. 2014 Jul;84(1):87-92. doi: 10.1016/j.urology.2014.04.014.
To reveal the impact of 2 biovars of Ureaplasma urealyticum on the sperm of infertile men by producing reactive oxygen species (ROS).
A total of 223 infertile and 146 fertile men were recruited into the study. Standard semen analysis was performed. Culturing and real-time polymerase chain reaction were used to identify biovars of U urealyticum in the semen. Semen ROS, malondialdehyde, and total superoxide dismutase levels were measured. Sperm nuclear deoxyribonucleic acid (DNA) damage was assessed of by sperm chromatin structure assay and single-cell gel electrophoresis.
Biovar II infection was more frequent in infertile men (P=.036). When compared with uninfected individuals, biovar II-infected men displayed decreases in spermatozoa concentration (P=.041); biovar II and mix-infected men displayed decreases in total motility (P=.015; P=.014, respectively) and increase in leukocyte counts (P=.004; P=.003, respectively). Except for total superoxide dismutase level, indicators for peroxide including ROS level, malondialdehyde level, DNA fragmentation index and high DNA stainable in sperm chromatin structure assay, and tail moment in single-cell gel electrophoresis exhibited the significant differences between both infected groups vs the uninfected group (P<05).
Compared with biovar I, biovar II is more likely to cause male infertility. Increased leukocyte counts, ROS level elevation, and subsequent spermatozoa membrane and DNA damage may be involved in this pathogenesis.
通过产生活性氧（ROS）来揭示脲原体 2 种生物变体对不孕男性精子的影响。
共招募 223 名不孕男性和 146 名生育男性参与研究。进行标准精液分析。采用培养和实时聚合酶链反应鉴定精液中 U 脲原体的生物变体。测量精液 ROS、丙二醛和总超氧化物歧化酶水平。通过精子染色质结构分析和单细胞凝胶电泳评估精子核脱氧核糖核酸（DNA）损伤。
生物变体 II 感染在不孕男性中更为常见（P=.036）。与未感染者相比，生物变体 II 感染者的精子浓度降低（P=.041）；生物变体 II 和混合感染者的总活力降低（P=.015；P=.014），白细胞计数增加（P=.004；P=.003）。除总超氧化物歧化酶水平外，ROS 水平、丙二醛水平、DNA 片段化指数、精子染色质结构分析中的高 DNA 可染性和单细胞凝胶电泳中的尾部矩等过氧化物指标在两组感染者与未感染者之间均存在显著差异（P<0.05）。
与生物变体 I 相比，生物变体 II 更可能导致男性不育。白细胞计数增加、ROS 水平升高以及随后的精子膜和 DNA 损伤可能参与了这种发病机制。
