Bostancı Mehmet Sühha, Bayram Merih, Bakacak Süleyman Murat, Yıldırım Ozge Kızılkale, Attar Rukset, Yıldırım Gazi, Bağrıaçık Emin Ümit, Celtemen Baran
Department of Obstetrics and Gynecology, Sakarya University Faculty of Medicine, Sakarya, Turkey.
Department of Obstetrics and Gynecology, Gazi University Faculty of Medicine, Ankara, Turkey.
J Turk Ger Gynecol Assoc. 2014 Jun 1;15(2):92-5. doi: 10.5152/jtgga.2014.13005. eCollection 2014.
The aim of this study is investigate the role of the Twist homolog 1 (TWIST), serine peptidase inhibitor (SERPINB5), and plasminogen activator inhibitor 1 (SERPIN1) genes in uterine leiomyoma etiopathogenesis.
Twelve patients, aged between 39 and 58, and had a hysterectomy, were included in the study. The size of the leiomyomas was between 20 and 130 mm based on gross pathology after hysterectomy. Tissue samples were obtained from normal myometrium and leiomyoma (1 cm(3)) tissue of the uterus of the patients and stored at -86°C. Samples were divided to two groups after histopathological evaluation of the uterus: normal myometrial tissues as control group (Group 1) and leiomyoma tissue as the study group (Group 2). The TWIST, SERPINB5, and SERPIN1 genes were studied for uterine leiomyoma etiopathogenesis.
TWIST gene expression was significantly higher in the uterine leiomyoma tissue (p<0.001). SERPINB5 and SERPIN1 gene expression was decreased in the uterine leiomyoma tissue, but the differences were not statistically significant.
TWIST gene activity is significantly increased in leiomyoma tissue when compared to normal myometrium. In spite of the fact that the development of uterine leiomyomas is estrogen- and progesterone-dependent, myometrial cells could be triggered by the TWIST gene for uterine leiomyoma development.
本研究旨在探讨 Twist 同源物 1（TWIST）、丝氨酸蛋白酶抑制剂（SERPINB5）和纤溶酶原激活物抑制剂 1（SERPIN1）基因在子宫平滑肌瘤发病机制中的作用。
本研究纳入了 12 例年龄在 39 至 58 岁之间且接受了子宫切除术的患者。根据子宫切除术后大体病理，平滑肌瘤大小在 20 至 130 毫米之间。从患者子宫的正常肌层和平滑肌瘤（1 立方厘米）组织中获取组织样本，并储存在 -86°C。在对子宫进行组织病理学评估后，将样本分为两组：正常肌层组织作为对照组（第 1 组），平滑肌瘤组织作为研究组（第 2 组）。研究 TWIST、SERPINB5 和 SERPIN1 基因在子宫平滑肌瘤发病机制中的作用。
TWIST 基因在子宫平滑肌瘤组织中的表达显著更高（p<0.001）。SERPINB5 和 SERPIN1 基因在子宫平滑肌瘤组织中的表达降低，但差异无统计学意义。
与正常肌层相比，TWIST 基因活性在平滑肌瘤组织中显著增加。尽管子宫平滑肌瘤的发生依赖于雌激素和孕激素，但 TWIST 基因可能触发肌层细胞导致子宫平滑肌瘤的发生。
