Reeve Wayne, Nandasena Kemanthi, Yates Ron, Tiwari Ravi, O'Hara Graham, Ninawi Mohamed, Chertkov Olga, Goodwin Lynne, Bruce David, Detter Chris, Tapia Roxanne, Han Shunseng, Woyke Tanja, Pitluck Sam, Nolan Matt, Land Miriam, Copeland Alex, Liolios Konstantinos, Pati Amrita, Mavromatis Konstantinos, Markowitz Victor, Kyrpides Nikos, Ivanova Natalia, Goodwin Lynne, Meenakshi Uma, Howieson John
Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia ; Department of Agriculture and Food, Western Australia, Australia.
Stand Genomic Sci. 2013 Dec 15;9(2):294-303. doi: 10.4056/sigs.4538264. eCollection 2013 Dec 20.
Mesorhizobium opportunistum strain WSM2075(T) was isolated in Western Australia in 2000 from root nodules of the pasture legume Biserrula pelecinus that had been inoculated with M. ciceri bv. biserrulae WSM1271. WSM2075(T) is an aerobic, motile, Gram negative, non-spore-forming rod that has gained the ability to nodulate B. pelecinus but is completely ineffective in N2 fixation with this host. This report reveals that the genome of M. opportunistum strain WSM2075(T) contains a chromosome of size 6,884,444 bp, encoding 6,685 protein-coding genes and 62 RNA-only encoding genes. The genome contains no plasmids, but does harbor a 455.7 kb genomic island from Mesorhizobium ciceri bv. biserrulae WSM1271 that has been integrated into a phenylalanine-tRNA gene.
机会中慢生根瘤菌菌株WSM2075(T)于2000年在西澳大利亚从已接种鹰嘴豆生物型双小扁豆根瘤菌WSM1271的豆科牧草双小扁豆的根瘤中分离得到。WSM2075(T)是一种需氧、具运动性、革兰氏阴性、不形成芽孢的杆菌,它获得了使双小扁豆结瘤的能力,但在用该宿主进行固氮时完全无效。本报告显示,机会中慢生根瘤菌菌株WSM2075(T)的基因组包含一条大小为6,884,444 bp的染色体,编码6,685个蛋白质编码基因和62个仅编码RNA的基因。该基因组不含质粒,但确实含有一个来自鹰嘴豆生物型双小扁豆根瘤菌WSM1271的455.7 kb基因组岛,该基因组岛已整合到一个苯丙氨酸 - tRNA基因中。