Patel U D, Govindarajan P, Dave P J
Department of Microbiology, Faculty of Science, M.S. University of Baroda, India.
Appl Environ Microbiol. 1989 Feb;55(2):465-7. doi: 10.1128/aem.55.2.465-467.1989.
Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same system. Degradation of aflatoxin B1 was confirmed by high-pressure liquid chromatographic and thin-layer chromatographic analysis. Ames microsomal mutagenicity test showed loss of aflatoxin activity. This method of detoxification also reduces the toxin levels effectively in artificially contaminated groundnuts.
利用γ射线和过氧化氢对黄曲霉毒素B1的灭活进行了研究。在5%过氧化氢存在的情况下，100千拉德的γ射线剂量足以使50微克黄曲霉毒素B1失活，而在同一系统中，100微克黄曲霉毒素的完全降解需要400千拉德。通过高压液相色谱和薄层色谱分析证实了黄曲霉毒素B1的降解。艾姆斯微粒体诱变性试验表明黄曲霉毒素活性丧失。这种解毒方法也能有效降低人工污染花生中的毒素水平。
