Chinchaladze D Z, Prangishvili D A, Scamrov A V, Beabealashvili R S, Dyatkina N B, Krayevsky A A
Institute of Molecular Biology and Biological Physics, Georgian SSR Academy of Sciences, Tbilisi, U.S.S.R.
Biochim Biophys Acta. 1989 Jun 1;1008(1):113-5. doi: 10.1016/0167-4781(89)90177-2.
The ability of a wide variety of nucleoside 5'-triphosphates with modified sugar moiety to serve as substrates in DNA synthesis catalyzed by DNA polymerase A from the archaebacterium Sulfolobus acidocaldarius was studied. Most of the dNTP analogs tested are shown to be specific terminating substrates for the synthesis irreversibly blocking further elongation of a nascent chain. The most powerful inhibitors were found to be 3'-amino derivatives of deoxy and arabino nucleoside triphosphates, while specific reverse transcriptase inhibitors, 3'-azido and 3'-methoxy derivatives of dNTP, were found to be inactive.
研究了多种具有修饰糖部分的核苷5'-三磷酸作为嗜热栖热菌DNA聚合酶A催化的DNA合成底物的能力。所测试的大多数dNTP类似物被证明是合成的特异性终止底物，不可逆地阻断新生链的进一步延伸。发现最有效的抑制剂是脱氧核苷三磷酸和阿拉伯糖核苷三磷酸的3'-氨基衍生物，而特定的逆转录酶抑制剂，即dNTP的3'-叠氮基和3'-甲氧基衍生物，则没有活性。
