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基于复用技术的离轴数字全息术中的实时定量相位重建

Real-time quantitative phase reconstruction in off-axis digital holography using multiplexing.

作者信息

Girshovitz Pinhas, Shaked Natan T

出版信息

Opt Lett. 2014 Apr 15;39(8):2262-5. doi: 10.1364/OL.39.002262.

DOI:10.1364/OL.39.002262
PMID:24978968
Abstract

We present a new approach for obtaining significant speedup in the digital processing of extracting unwrapped phase profiles from off-axis digital holograms. The new technique digitally multiplexes two orthogonal off-axis holograms, where the digital reconstruction, including spatial filtering and two-dimensional phase unwrapping on a decreased number of pixels, can be performed on both holograms together, without redundant operations. Using this technique, we were able to reconstruct, for the first time to our knowledge, unwrapped phase profiles from off-axis holograms with 1 megapixel in more than 30 frames per second using a standard single-core personal computer on a MATLAB platform, without using graphic-processing-unit programming or parallel computing. This new technique is important for real-time quantitative visualization and measurements of highly dynamic samples and is applicable for a wide range of applications, including rapid biological cell imaging and real-time nondestructive testing. After comparing the speedups obtained by the new technique for holograms of various sizes, we present experimental results of real-time quantitative phase visualization of cells flowing rapidly through a microchannel.

摘要

我们提出了一种新方法,可在从离轴数字全息图中提取展开相位轮廓的数字处理过程中显著加快速度。这项新技术对两个正交离轴全息图进行数字复用,这样包括空间滤波和在较少像素数量上进行二维相位展开的数字重建,就可以在两个全息图上同时进行,而无需进行冗余操作。据我们所知,使用这种技术,我们首次能够在MATLAB平台上使用标准单核个人计算机,每秒30多帧地从具有100万像素的离轴全息图中重建展开相位轮廓,而无需使用图形处理单元编程或并行计算。这项新技术对于高动态样本的实时定量可视化和测量非常重要,并且适用于广泛的应用,包括快速生物细胞成像和实时无损检测。在比较了新技术对各种尺寸全息图所获得的加速效果后,我们展示了细胞快速流过微通道时实时定量相位可视化的实验结果。

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