Poland Simon P, Krstajić Nikola, Knight Robert D, Henderson Robert K, Ameer-Beg Simon M
Opt Lett. 2014 Apr 15;39(8):2431-4. doi: 10.1364/OL.39.002431.
We report on the development of a doubly weighted Gerchberg-Saxton algorithm (DWGS) to enable generation of uniform beamlet arrays with a spatial light modulator (SLM) for use in multiphoton multifocal imaging applications. The algorithm incorporates the WGS algorithm as well as feedback of fluorescence signals from the sample measured with a single-photon avalanche diode (SPAD) detector array. This technique compensates for issues associated with nonuniform illumination onto the SLM, the effects due to aberrations and the variability in gain between detectors within the SPAD array to generate a uniformly illuminated multiphoton fluorescence image. We demonstrate the use of the DWGS with a number of beamlet array patterns to image muscle fibers of a 5-day-old fixed zebrafish larvae.
我们报告了一种双加权格尔奇贝格-萨克斯顿算法（DWGS）的开发，该算法能够利用空间光调制器（SLM）生成均匀的子束阵列，用于多光子多焦点成像应用。该算法结合了WGS算法以及通过单光子雪崩二极管（SPAD）探测器阵列测量的来自样品的荧光信号反馈。这种技术补偿了与SLM上不均匀照明相关的问题、像差的影响以及SPAD阵列内探测器之间增益的变化，以生成均匀照明的多光子荧光图像。我们展示了DWGS与多种子束阵列模式一起用于对5天大的固定斑马鱼幼虫的肌肉纤维进行成像。
