Zhang Yuanyuan, Gao Rui, Liu Min, Yan Changjiang, Shan Anshan
a Institute of Animal Nutrition , Northeast Agricultural University , Harbin , P.R. China.
Arch Anim Nutr. 2014;68(4):320-35. doi: 10.1080/1745039X.2014.927710. Epub 2014 Jun 30.
The aim of the present study was to investigate the sorption properties in vitro and the application feasibility of modified halloysite nanotubes (HNT) in reducing the toxic effect of ZEN in rats in vivo. HNT were modified using the surfactant stearyldimethylbenzylammonium chloride. Modified HNT (MHNT) was evaluated using electron microscopy, which revealed that the modification had successfully enlarged the nanotube inner diameter from 11.35 to 20.12 nm. In an in vitro study, the efficiency of MHNT to adsorb zearalenone (ZEN) from simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) was investigated in comparison with HNT or a montmorillonite mixture (MON). For all tested adsorbents, the adsorption efficiency for ZEN was increased when the adsorbent dose rose from 0.4 to 1.0 mg/ml and became stable beyond this dose. Moreover, the ZEN adsorption increased with the extension of adsorption times from 20 to 90 min (SGF) and from 30 to 120 min (SIF) without further increase afterwards. Already at the first measuring times (20 and 30 min for SGF and SIF, respectively) MHNT showed a higher adsorptive property then HNT. The ability of MHNT to prevent lesions caused by ZEN was evaluated in 60 female rats. The rats received five experimental diets for two weeks: Control (per kg diet 0.001 mg ZEN); ZEN (0.5 mg ZEN), HNT (0.5 mg ZEN + 1% HNT), MHNT (0.5 mg ZEN + 1% MHNT) and MON (0.5 mg ZEN + 1% MON). The results indicated that the tested adsorbents mitigated toxic and estrogenic effects of ZEN exposure including changes in oxidative stress biomarkers and organ weights. In some parameters (gain, oestradiol content in serum and ZEN concentration in reproductive organs), MHNT exceeded the effectiveness of HNT. Thus, it can be concluded that the modification enhances the adsorbent properties of HNT and that MHNT can bind to ZEN in animal feed or in the gastrointestinal tract.
本研究的目的是调查改性埃洛石纳米管（HNT）的体外吸附特性及其在降低大鼠体内玉米赤霉烯酮（ZEN）毒性作用方面的应用可行性。使用表面活性剂硬脂基二甲基苄基氯化铵对HNT进行改性。通过电子显微镜对改性HNT（MHNT）进行评估，结果显示改性成功地将纳米管内径从11.35nm扩大到了20.12nm。在一项体外研究中，将MHNT与HNT或蒙脱石混合物（MON）相比较，研究其从模拟胃液（SGF）和模拟肠液（SIF）中吸附玉米赤霉烯酮（ZEN）的效率。对于所有测试的吸附剂，当吸附剂剂量从0.4mg/ml增加到1.0mg/ml时，ZEN的吸附效率提高，超过此剂量后趋于稳定。此外，随着吸附时间从20分钟延长至90分钟（SGF）以及从30分钟延长至120分钟（SIF），ZEN的吸附量增加，之后不再进一步增加。在最初的测量时间（SGF和SIF分别为20分钟和30分钟），MHNT就显示出比HNT更高的吸附性能。在60只雌性大鼠中评估了MHNT预防ZEN所致损伤的能力。大鼠接受五种实验性日粮，持续两周：对照组（每千克日粮含0.001mg ZEN）；ZEN组（0.5mg ZEN）、HNT组（0.5mg ZEN + 1% HNT）、MHNT组（0.5mg ZEN + 1% MHNT）和MON组（0.5mg ZEN + 1% MON）。结果表明，所测试的吸附剂减轻了ZEN暴露的毒性和雌激素效应，包括氧化应激生物标志物和器官重量的变化。在一些参数方面（体重增加、血清雌二醇含量和生殖器官中的ZEN浓度），MHNT的效果超过了HNT。因此，可以得出结论，改性增强了HNT的吸附性能，并且MHNT可以在动物饲料或胃肠道中与ZEN结合。
