印记基因表达和 DNA 甲基化状态在猪孤雌生殖胚胎和胎盘中的破坏。
Disruption of imprinted gene expression and DNA methylation status in porcine parthenogenetic fetuses and placentas.
机构信息
Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China.
Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China.
出版信息
Gene. 2014 Sep 1;547(2):351-8. doi: 10.1016/j.gene.2014.06.059. Epub 2014 Jun 27.
Parthenogenetically activated oocytes cannot develop to term in mammals due to the lack of paternal gene expression and failed X chromosome inactivation (XCI). To further characterize porcine parthenogenesis, the expression of 18 imprinted genes was compared between parthenogenetic (PA) and normally fertilized embryos (Con) using quantitative real-time PCR (qRT-PCR). The results revealed that maternally expressed genes were over-expressed, whereas paternally expressed genes were significantly reduced in PA fetuses and placentas. The results of bisulfite sequencing PCR (BSP) demonstrated that PRE-1 and Satellite were hypermethylated in both Con and PA fetuses and placentas, while XIST DMRs were hypomethylated only in PA samples. Taken together, these results suggest that the aberrant methylation profile of XIST DMRs and abnormal imprinted gene expression may be responsible for developmental failure and impaired growth in porcine parthenogenesis.
由于缺乏父本基因表达和 X 染色体失活（XCI）失败，孤雌激活的卵母细胞在哺乳动物中无法发育到足月。为了进一步表征猪孤雌生殖，我们使用定量实时 PCR（qRT-PCR）比较了孤雌生殖（PA）和正常受精胚胎（Con）之间 18 个印迹基因的表达。结果表明，母源表达基因过度表达，而父源表达基因在 PA 胎儿和胎盘中显著减少。亚硫酸氢盐测序 PCR（BSP）的结果表明，在 Con 和 PA 胎儿和胎盘中，PRE-1 和 Satellite 均呈超甲基化，而 XIST DMR 仅在 PA 样本中呈低甲基化。综上所述，这些结果表明，XIST DMRs 的异常甲基化谱和异常印迹基因表达可能是猪孤雌生殖发育失败和生长受损的原因。
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