Wang Dongxu, Liu Zhiquan, Yao Haobin, Hao Yang, Zhou Lina, Du Jian, Zhu Yixin, Xu Yuxin, Wang Guodong, Song Yuning, Li Zhanjun
College of Animal Science, Jilin University, Changchun 130062, China.
College of Animal Science, Jilin University, Changchun 130062, China.
Gene. 2017 Aug 30;626:158-162. doi: 10.1016/j.gene.2017.05.035. Epub 2017 May 16.
Parthenogenetically activated oocytes cannot develop to term in mammals due to lack of paternal gene expression. Disruption of imprinted gene expression and DNA methylation status in parthenogenetic fetuses has been reported in mice and pigs, but not in rabbits. In this study, the genomic imprinting status of the paternally expressed genes Neuronatin (NNAT), Nucleosome assembly protein 1-like 5 (NAP1L5), and Makorin ring finger protein 3 (MKRN3) was compared between rabbit parthenogenetic (PA) and normally fertilized fetuses (Con) using quantitative real-time PCR (qRT-PCR) and bisulfite sequencing PCR (BSP). The results revealed a significantly reduced expression of NNAT, NAP1L5, and MKRN3 in rabbit PA fetuses compared with Con fetuses (p<0.05). In addition, the BSP results demonstrated hypermethylation in the differentially methylated regions (DMRs) of NNAT, NAP1L5, and MKRN3 in rabbit PA fetuses. Taken together, these results suggest that hypermethylation of DMRs is associated with decreased NNAT, NAP1L5, and MKRN3 expression, which may be responsible for developmental failure of rabbit PA fetuses.
由于缺乏父本基因表达，孤雌生殖激活的卵母细胞在哺乳动物中无法发育至足月。在小鼠和猪中已报道了孤雌生殖胎儿中印迹基因表达和DNA甲基化状态的破坏，但在兔子中尚未见报道。在本研究中，使用定量实时PCR（qRT-PCR）和亚硫酸氢盐测序PCR（BSP）比较了兔子孤雌生殖（PA）胎儿和正常受精胎儿（Con）中父本表达基因神经生长素（NNAT）、核小体组装蛋白1样5（NAP1L5）和马科林环指蛋白3（MKRN3）的基因组印记状态。结果显示，与Con胎儿相比，兔子PA胎儿中NNAT、NAP1L5和MKRN3的表达显著降低（p<0.05）。此外，BSP结果表明兔子PA胎儿中NNAT、NAP1L5和MKRN3的差异甲基化区域（DMRs）存在高甲基化。综上所述，这些结果表明DMRs的高甲基化与NNAT、NAPI L5和MKRN3表达降低有关，这可能是兔子PA胎儿发育失败的原因。
