Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, China.
Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
Gene. 2014 Sep 1;547(2):288-94. doi: 10.1016/j.gene.2014.06.061. Epub 2014 Jun 27.
TBC1D1 plays an important role in numerous fundamental physiological processes including muscle metabolism, regulation of whole body energy homeostasis and lipid metabolism. The objective of the present study was to identify single nucleotide polymorphisms (SNPs) in chicken TBC1D1 using 128 Erlang mountainous chickens and to determine if these SNPs are associated with carcass traits. The approach consisted of sequencing TBC1D1 using a panel of DNA from different individuals, revealing twenty-two SNPs. Among these SNPs, two polymorphisms (g.69307744C>T and g.69307608T>G) of block 1, four polymorphisms (g.69322320C>T, g.69322314G>A, g.69317290A>G and g.69317276T>C) of block 2 and four polymorphisms of block 3 (g.69349746G>A, g.69349736C>G, g.69349727C>T and g.69349694C>T) exhibited a high degree of linkage disequilibrium in all test populations. An association analysis was performed between the twenty-two SNPs and seven performance traits. SNPs g.69307744C>T, g.69340192G>A and g.69355665T>C were demonstrated to have a strong effect on liveweight (BW), carcass weight (CW), semi-eviscerated weight (SEW) and eviscerated weight (EW) and g.69340070C>T polymorphism was related to BW, SEW and BMW in chicken populations. However, for the other SNPs, there were no significant correlations between different genotypes and carcass traits. Meanwhile, haplotype CT-TG of block 1 and combined genotype AG-TT-AC-CT of block 3 were significantly associated with BW, CW, SEW and EW. Overall, our results provide evidence that polymorphisms in TBC1D1 are associated with carcass traits and would be a useful candidate gene in selection programs for improving carcass traits.
TBC1D1 在许多基本生理过程中发挥着重要作用，包括肌肉代谢、全身能量稳态调节和脂质代谢。本研究的目的是使用 128 只二郎山鸡鉴定鸡 TBC1D1 中的单核苷酸多态性（SNP），并确定这些 SNP 是否与胴体性状相关。该方法包括使用不同个体的 DNA 面板对 TBC1D1 进行测序，揭示了 22 个 SNP。在这些 SNP 中，1 号块的两个多态性（g.69307744C>T 和 g.69307608T>G）、2 号块的四个多态性（g.69322320C>T、g.69322314G>A、g.69317290A>G 和 g.69317276T>C）和 3 号块的四个多态性（g.69349746G>A、g.69349736C>G、g.69349727C>T 和 g.69349694C>T）在所有测试群体中表现出高度的连锁不平衡。对 22 个 SNP 与 7 个性能性状进行了关联分析。SNP g.69307744C>T、g.69340192G>A 和 g.69355665T>C 被证明对活重（BW）、胴体重（CW）、半净膛重（SEW）和净膛重（EW）有很强的影响，g.69340070C>T 多态性与 BW、SEW 和 BMW 有关鸡群。然而，对于其他 SNP，不同基因型与胴体性状之间没有显著相关性。同时，1 号块的 CT-TG 单倍型和 3 号块的 AG-TT-AC-CT 组合基因型与 BW、CW、SEW 和 EW 显著相关。总体而言，我们的研究结果提供了证据表明，TBC1D1 中的多态性与胴体性状相关，并且可能成为提高胴体性状选择计划的有用候选基因。
