Allenby C F, Basketter D A
Lister Hospital, Stevenage, Hertfordshire, UK.
Contact Dermatitis. 1989 Mar;20(3):185-90. doi: 10.1111/j.1600-0536.1989.tb04655.x.
In sensitized subjects, minimum eliciting levels of cobalt were estimated using patch tests with aqueous cobalt chloride on both normal skin and on skin pretreated in various ways to enhance penetration and reactivity. 6 reacted to 10,000 ppm and 1 gave an equivocal reaction to 1000 ppm aq. cobalt. Pretreatment of the patch test site for 24 h with surfactant enhanced reactivity, reducing the minimum eliciting concentration to 1000 ppm cobalt chloride in 1 subject, to 100 ppm in 2 subjects, and in 3 subjects to 10 ppm aqueous cobalt chloride. No reactions were obtained at 1 ppm. EDTA was effective in reducing the response to aqueous cobalt.
在致敏受试者中，通过在正常皮肤以及以各种方式预处理以增强渗透性和反应性的皮肤上进行氯化钴水溶液的斑贴试验，来估计引发钴反应的最低水平。6人对10,000 ppm有反应，1人对1000 ppm氯化钴水溶液有可疑反应。用表面活性剂对斑贴试验部位预处理24小时可增强反应性，使1名受试者引发反应的最低浓度降至1000 ppm氯化钴，2名受试者降至100 ppm，3名受试者降至10 ppm氯化钴水溶液。1 ppm时未出现反应。乙二胺四乙酸（EDTA）可有效降低对氯化钴水溶液的反应。
