Fischer T, Maibach H
Contact Dermatitis. 1984 Nov;11(5):285-7. doi: 10.1111/j.1600-0536.1984.tb01011.x.
The concentration of nickel sulphate was determined in 9-16 mg test samples from commercially available patch test antigens. With two different manufacturers of 2.5% nickel sulphate in pet, the concentration range of 25 samples was 0.7 and 1.1% (+/- 2 SD) respectively, and with one manufacturer of 5.0% nickel sulphate in pet, the range was +/- 0.5%. In a normal patch test, the volume of patch test material applied with the Finn chamber technique should be 12-18 microliter (9-16 mg), but has been found to range between 9 and 50 microliter. Thus the amount of nickel applied in standard patch tests varies within at least 6-fold limits.
对市售斑贴试验抗原中9 - 16毫克的测试样品测定了硫酸镍的浓度。对于两家不同生产商生产的含2.5%硫酸镍的凡士林制剂，25个样品的浓度范围分别为0.7%和1.1%（±2标准差），而对于一家生产含5.0%硫酸镍凡士林制剂的生产商，浓度范围为±0.5%。在常规斑贴试验中，采用芬兰小室技术应用的斑贴试验材料体积应为12 - 18微升（9 - 16毫克），但已发现其范围在9至50微升之间。因此，标准斑贴试验中应用的镍含量变化至少在6倍的范围内。
