母菊天蓝烃的抗氧化及自由基清除活性。
Antioxidant and radical scavenging activities of chamazulene.
作者信息
Capuzzo Andrea, Occhipinti Andrea, Maffei Massimo E
机构信息
a Biosfered S.r.l., Academic Spin-Off of the University of Turin , Via G. Quarello 15/A, 10135 Turin , Italy.
出版信息
Nat Prod Res. 2014;28(24):2321-3. doi: 10.1080/14786419.2014.931393. Epub 2014 Jul 1.
Essential oils (EOs) of chamomile contain several bioactive compounds, including monoterpenes, sesquiterpenes, triterpenes and fatty acids. Hydrodistillation of chamomile EO induces the formation of chamazulene, a bioactive compound. Chamazulene was isolated from the EO by column chromatography. The total antioxidant capacity confirmed a higher antioxidant activity of chamazulene (IC50 = 6.4 μg mL(- 1)) than of ascorbic acid (IC50 = 12.8 μg mL(- 1)), α-tocopherol (IC50 = 20.5 μg mL(- 1)) and of butylated hydroxytoluene (BHT) (IC50 = 30.8 μg mL(- 1)). Chamazulene was unable to react with DPPH√. However, when chamazulene was assayed with ABTS√, a strong and significantly (P < 0.05) higher free radical scavenging activity was observed (IC50 = 3.7 μg mL(- 1)), with respect to BHT (IC50 = 6.2 μg mL(- 1)) and α-tocopherol (IC50 = 11.5 μg mL(- 1)). The results of this work show that chamazulene is an important factor for the antioxidant power of chamomile oil.
洋甘菊精油含有多种生物活性化合物，包括单萜类、倍半萜类、三萜类和脂肪酸。通过水蒸馏法提取洋甘菊精油会诱导形成一种生物活性化合物——蓝香油烃。通过柱色谱法从精油中分离出蓝香油烃。总抗氧化能力证实，蓝香油烃（IC50 = 6.4 μg mL(-1)）的抗氧化活性高于抗坏血酸（IC50 = 12.8 μg mL(-1)）、α-生育酚（IC50 = 20.5 μg mL(-1)）和丁基化羟基甲苯（BHT）（IC50 = 30.8 μg mL(-1)）。蓝香油烃无法与二苯基苦味酰基自由基（DPPH√）反应。然而，当用2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS√）对蓝香油烃进行检测时，观察到其具有较强且显著（P < 0.05）更高的自由基清除活性（IC50 = 3.7 μg mL(-1)），相对于BHT（IC50 = 6.2 μg mL(-1)）和α-生育酚（IC50 = 11.5 μg mL(-1)）。这项工作的结果表明，蓝香油烃是洋甘菊油抗氧化能力的一个重要因素。
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