Rekka E A, Kourounakis A P, Kourounakis P N
Department of Pharmaceutical Chemistry, School of Pharmacy, Aristotelian University of Thessaloniki, Greece.
Res Commun Mol Pathol Pharmacol. 1996 Jun;92(3):361-4.
Oxygen toxicity and related free radical reactions are implicated in numerous pathophysiological conditions, like atherosclerosis, inflammation, gastric ulceration, neuronal degeneration, tumour promotion. The flowers of Matricaria chamomilla, Asteraceae, have been used therapeutically for conditions in which oxidative stress is supposed to be implicated. We considered interesting to investigate the effect of Chamazulene, the active substance of chamomile, on free radical processes. Membrane lipid peroxidation was induced by Fe2+/ascorbate and assessed as the 2-thiobarbituric acid reactive material. The hydroxyl radical scavenging activity was studied as the competition of Chamazulene with DMSO for HO. generated by Fe3+/ascorbate. Finally, the interaction of Chamazulene with the N-centered stable free radical DPPH was estimated photometrically (517 nm). It was found that Chamazulene inhibited lipid peroxidation in a concentration and time dependent manner presenting an IC50 of 18 microM after 45 min incubation. It could also inhibit the autoxidation of DMSO (33 mM) by 76% at 25 mM, and had a weak capacity to interact with DPPH. In conclusion, Chamazulene presents interesting properties concerning radical processes.
氧中毒及相关自由基反应与多种病理生理状况有关，如动脉粥样硬化、炎症、胃溃疡、神经元变性、肿瘤进展。菊科植物母菊花的花朵已被用于治疗被认为与氧化应激有关的病症。我们认为研究母菊花的活性物质天蓝烃对自由基过程的影响很有意思。膜脂质过氧化由Fe2+/抗坏血酸诱导，并作为2-硫代巴比妥酸反应性物质进行评估。通过研究天蓝烃与二甲基亚砜（DMSO）竞争由Fe3+/抗坏血酸产生的羟基自由基（HO·），来考察其清除羟基自由基的活性。最后，通过光度法（517nm）测定天蓝烃与以氮为中心的稳定自由基二苯基苦味酰基自由基（DPPH）的相互作用。结果发现，天蓝烃以浓度和时间依赖性方式抑制脂质过氧化，孵育45分钟后IC50为18微摩尔。在25毫摩尔浓度下，它还能抑制二甲基亚砜（33毫摩尔）的自氧化达76% ，并且与二苯基苦味酰基自由基（DPPH）相互作用的能力较弱。总之，天蓝烃在自由基过程方面表现出有趣的特性。
