Halstensen T S, Mollnes T E, Brandtzaeg P
Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), University of Oslo, National Hospital, Norway.
Gastroenterology. 1989 Jul;97(1):10-9. doi: 10.1016/0016-5085(89)91409-1.
Extensively washed and ethanol-fixed colonic specimens from 10 patients with ulcerative colitis, 3 patients with Crohn's disease of the colon, and 8 histologically normal controls were examined by two-color immunohistochemistry with monoclonal antibody to a neoepitope in the terminal complement complex combined with antiserum to factor VIII-related antigen (von Willebrand's factor), C3c, C3d, or C5. An alternative combination was monoclonal antibody to S-protein and antiserum to C9. Submucosal vessel walls in both normal and diseased colon showed parallel positivity for C3d, C5, C9, terminal complement complex, and S-protein, but the staining intensity and the proportion of positive vessels were significantly higher in inflammatory bowel disease than in controls. In addition, there was significantly more C3c reactivity associated with the terminal complement complex-positive submucosal vessels of active inflammatory bowel disease lesions than in histologically normal colon. Vascular C activation may therefore be a continuous process in active inflammatory bowel disease lesions, presumably related to the degree of inflammation and immune complex formation.
对10例溃疡性结肠炎患者、3例结肠克罗恩病患者以及8例组织学正常对照者的结肠标本进行广泛冲洗并乙醇固定，采用针对末端补体复合物中新表位的单克隆抗体与抗VIII因子相关抗原（血管性血友病因子）、C3c、C3d或C5的抗血清进行双色免疫组织化学检测。另一种组合是抗S蛋白单克隆抗体和抗C9抗血清。正常结肠和病变结肠的黏膜下血管壁对C3d、C5、C9、末端补体复合物和S蛋白均呈平行阳性，但炎症性肠病中染色强度和阳性血管比例显著高于对照组。此外，与组织学正常结肠相比，活动性炎症性肠病病变的末端补体复合物阳性黏膜下血管中C3c反应性显著更多。因此，血管C激活可能是活动性炎症性肠病病变中的一个持续过程，可能与炎症程度和免疫复合物形成有关。
