Halstensen T S, Mollnes T E, Fausa O, Brandtzaeg P
Institute of Pathology, National Hospital, Rikshospitalet, Oslo, Norway.
Gut. 1989 Mar;30(3):361-6. doi: 10.1136/gut.30.3.361.
Extensively washed, ethanol fixed and paraffin embedded colonic specimens from 15 patients with ulcerative colitis (UC) and nine patients with Crohn's disease (CD) of the colon, ileal specimens from six patients with CD of the ileum, and histologically normal control specimens obtained from 10 patients operated for colonic carcinoma, were examined by immunohistochemistry with a monoclonal antibody specific for a neoepitope in the C9 part of the terminal complement complex (TCC). The submucosal blood vessels in inflammatory bowel disease (IBD) showed significantly more TCC positivity than the controls, and vascular TCC deposition was statistically related (p less than 0.001) to degree of inflammation. Five of the six ileal CD specimens contained likewise vascular TCC deposits. In addition, five UC specimens and one colonic CD specimen contained TCC-positive fibrils in the muscularis mucosae or submucosa. There was no significant difference in vascular TCC deposits between UC and CD. The results suggested that terminal complement activation takes place in the intestinal lesions of IBD.
对15例溃疡性结肠炎（UC）患者、9例结肠克罗恩病（CD）患者的结肠标本进行广泛冲洗、乙醇固定及石蜡包埋，对6例回肠克罗恩病患者的回肠标本，以及10例因结肠癌接受手术患者的组织学正常对照标本，采用针对末端补体复合物（TCC）C9部分新表位的单克隆抗体进行免疫组织化学检测。炎症性肠病（IBD）黏膜下血管的TCC阳性率显著高于对照组，血管TCC沉积与炎症程度在统计学上相关（p<0.001）。6例回肠CD标本中有5例同样存在血管TCC沉积。此外，5例UC标本和1例结肠CD标本在黏膜肌层或黏膜下层含有TCC阳性纤维。UC和CD之间的血管TCC沉积无显著差异。结果提示，IBD肠道病变中发生了末端补体激活。
