通过微细胞融合构建含单条人类染色体（X/常染色体易位）的小鼠A9克隆。
Construction of mouse A9 clones containing a single human chromosome (X/autosome translocation) via micro-cell fusion.
作者信息
Koi M, Morita H, Shimizu M, Oshimura M
机构信息
Laboratory of Cytogenetics, Kanagawa Cancer Center Research Institute.
出版信息
Jpn J Cancer Res. 1989 Feb;80(2):122-5. doi: 10.1111/j.1349-7006.1989.tb02278.x.
Cell hybrids between hypoxanthine guanine phosphoribosyl transferase (HGPRT)-deficient mouse cell lines (A9 or RAG) and each of 12 different human fibroblasts (GM cells) containing various X/autosome translocations were formed, selected and isolated. Several human chromosomes including an X/autosome translocation carrying HGPRT locus were found in these hybrid cells. To construct A9 cell clones that contain a single X/autosome translocation, micro-cell fusion was undertaken to transfer these chromosomes from the hybrids to A9 cells. Karyotype analysis revealed that most of the resulting micro-cell hybrids contain, in a background of mouse chromosomes, only the human X/autosome translocations which were present in the GM cells used for cell hybridization. Sublines of A9 cells were established containing the following autosomal segments: 1q23----1qter; 1q12----1pter; 3p12----3pter; 3q21----3qter; 11q13----11qter; 11q13----11pter; 11p11----11qter; 11q23----11pter; 12q24----12pter; 16q24----16pter; 17q11----17pter.
在次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）缺陷的小鼠细胞系（A9或RAG）与12种不同的含有各种X/常染色体易位的人类成纤维细胞（GM细胞）之间形成、筛选并分离出细胞杂种。在这些杂种细胞中发现了几条人类染色体，包括一条携带HGPRT基因座的X/常染色体易位。为构建含有单一X/常染色体易位的A9细胞克隆，进行了微细胞融合以将这些染色体从杂种细胞转移到A9细胞中。核型分析显示，大多数所得的微细胞杂种在小鼠染色体背景下仅含有用于细胞杂交的GM细胞中存在的人类X/常染色体易位。建立了含有以下常染色体片段的A9细胞亚系：1q23----1q末端；1q12----1p末端；3p12----3p末端；3q21----3q末端；11q13----11q末端；11q13----11p末端；11p11----11q末端；11q23----11p末端；12q24----12p末端；16q24----16p末端；17q11----17p末端。
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