Yamaguchi Y, Inaba K, Kawai J, Kato T, Nakamura S, Uno K, Muramatsu S
Department of Zoology, Faculty of Science, Kyoto University.
Jpn J Cancer Res. 1989 Feb;80(2):141-9. doi: 10.1111/j.1349-7006.1989.tb02282.x.
T cells prepared from tumor (Meth A)-bearing mice were cocultured with homologous tumor cells and splenic dendritic cells to enrich tumor-specific T cells by the separation of clusters. T blasts generated from clusters were capable of inhibiting the in vivo tumor cell growth. The culture supernatant of clustering cells (CLSN) was effective in activating macrophages (M phi) to be cytostatic and cytocidal against tumor cells. Moreover, it was found that CLSN contains at least 3 distinct factors; one was identified as interferon-gamma (IFN-gamma), and the others are so far unidentified, but one acts synergistically with IFN-gamma, possibly as the second signal, and the other cooperates with lipopolysaccharide but not with IFN-gamma. We propose that the tumor-specific T cells secrete soluble mediators which cooperate with each other in M phi activation against tumor cells.
从荷瘤（Meth A）小鼠制备的T细胞与同源肿瘤细胞和脾树突状细胞共培养，通过分离细胞簇来富集肿瘤特异性T细胞。从细胞簇产生的T母细胞能够抑制体内肿瘤细胞生长。细胞簇培养上清液（CLSN）可有效激活巨噬细胞（M phi），使其对肿瘤细胞具有细胞生长抑制和细胞杀伤作用。此外，发现CLSN至少含有3种不同因子；一种被鉴定为干扰素-γ（IFN-γ），其他因子目前尚未确定，但其中一种与IFN-γ协同作用，可能作为第二信号，另一种与脂多糖协同作用，但不与IFN-γ协同作用。我们提出，肿瘤特异性T细胞分泌可溶性介质，这些介质在激活巨噬细胞对抗肿瘤细胞的过程中相互协作。
